Detección de Tritrichomonas foetus por PCR en esmegma prepucial de toros en Uruguay

Q4 Veterinary

Veterinaria Pub Date : 2020-05-10 DOI:10.29155/VET.56.213.7

C. Silveira, Martín Fraga, Cecilia Monesiglio, R. Delpiazzo, Melissa Macías-Rioseco, Federico Giannitti, Franklin Riet-Correa

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引用次数: 1

Abstract

Bovine trichomoniasis is a venereal disease caused by Tritrichomonas foetus. It is responsible for economic losses worldwide, causing mainly infertility and abortion in cows and heifers. The diagnosis of this disease is mainly based on microbiological culture and on molecular testing. In Uruguay the parasite has not been reported since 2005. The aim of this study is to report the detection of T. foetus by PCR in preputial smegma from bulls from dairy and beef Uruguayan farms. A total of 310 smegma samples, 121 from dairy-production-bulls and 189 from meat-production-bulls, were sampled. Animals came from 56 farms distributed within 12 different departments of Uruguay. While the PCR analysis of preputial smegma identified 2.25% (7/310) positive for T. foetus, no culture-positive samples were detected. The parasite was detected in 5% (6/121) of the sampled bulls for dairy production, these six animals were from the same dairy farm. Tritrichomonas foetus was only detected in one out of 189 animals from beef production. This study showed that 3.4% (1/29) and 3.1% (1/32) of beef and dairy farms respectively, were positive for T. foetus infection. PCR is advantageous over culture; therefore, it is an efficient and quick method for the detection of T. foetus in preputial samples. The results of this work suggest that the parasite detection may be underestimated due to the use of only culture-based diagnostics.

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乌拉圭公牛包皮囊肿中胎儿三毛滴虫的PCR检测

牛毛滴虫病是由毛滴虫引起的一种性病。它在世界范围内造成经济损失，主要造成母牛和小母牛不孕和流产。本病的诊断主要依靠微生物培养和分子检测。在乌拉圭，这种寄生虫自2005年以来就没有报道过。本研究的目的是报告用聚合酶链反应(PCR)在乌拉圭奶牛和牛肉农场的公牛包皮包皮中检测到T.胎儿。总共采集了310个包皮垢样本，其中121个来自产奶公牛，189个来自产肉公牛。动物来自分布在乌拉圭12个不同省的56个农场。包皮包皮凝集膜PCR检测发现2.25% (7/310)T.胎儿阳性，未发现培养阳性样本。在奶牛生产公牛中，有5%(6/121)被检出寄生虫，这6头牛来自同一奶牛场。产牛肉的189只动物中，只有1只被检测出有胎毛滴虫。结果表明，牛场和奶牛场分别有3.4%(1/29)和3.1%(1/32)的牛场和奶牛场的T.胎儿感染呈阳性。PCR优于培养;因此，它是一种高效、快速的包皮样品中胎儿弓形虫的检测方法。这项工作的结果表明，由于只使用基于培养的诊断方法，寄生虫检测可能被低估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinaria 农林科学-兽医学

CiteScore

0.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： VETERINARIA is the official scientific journal of the Italian Companion Animal Veterinary Association (SCIVAC) and is published bimonthly by Edizioni Veterinarie (E.V.). Its aim is to promote the spread and development of new ideas and techniques in the field of clinical and veterinary practices, with the ultimate goal of improving and promoting the continuing education of veterinary practicioners. VETERINARIA publishes literature reviews, original articles, diagnostic corners and clinical cases on different topics related to medicine and surgery of the dog, cat and of other companion animals, as well as short communications from congresses.