Impact of Resveratrol in Attenuating Cisplatin Induced Testicular Toxicity in Male adult Rats

Dr. Shahnaz Memon, Dr. Sajjad Ali Almani, Dr. Muhammad Saqib Baloch, Dr. Samreen Ali, Tanveer Ahmed Talpur, Harender Kumar
{"title":"Impact of Resveratrol in Attenuating Cisplatin Induced Testicular Toxicity in Male adult Rats","authors":"Dr. Shahnaz Memon, Dr. Sajjad Ali Almani, Dr. Muhammad Saqib Baloch, Dr. Samreen Ali, Tanveer Ahmed Talpur, Harender Kumar","doi":"10.35787/jimdc.v11i1.716","DOIUrl":null,"url":null,"abstract":"Background and Objective: Resveratrol is a poly-hydroxyphenol plant toxin that alleviates oxidative stress by increasing endogenous antioxidant levels and prevents tissue damage. The study aimed to investigate the anti-oxidative role of Resveratrol by histochemical, ultrastructural, and biochemical methods in Cisplatin-induced testicular toxicity in Wistar Albino rats. \nMaterial and Methods: Quasi-experimental study was conducted at the Department of Pharmacology, Anatomy and Postgraduate Laboratory of ISRA University Hyderabad from October 2020 to March 2021. Twenty-four male, normal adult Wistar albino rats were recruited and distributed equally into; Group-A (Control), Group-B (Experimental group or Cisplatin group), Group-C (Experimental group or Cisplatin + Resveratrol combination group). Pre and post-experimental body weight, analysis of oxidative markers, semen parameters, and histomorphology were carried out in all three groups. SPSS version 24.0 was used for the analysis of Data. \nResults: A statistically significant decline in the body weight and testicular weight in group B and C respectively (p<0.05). While reduction in sperm count, motility and viability was observed in Group-B compared with Group-C (p<0.05). Oxidative markers were also significantly depleted in Group-B in comparison to Group-C (p<0.05). Evident changes were observed in the testicular histology of Group-B compared with Group-C (p<0.05). Irregular, regressive, and atrophic seminiferous tubules were seen in Group-B. Most seminiferous tubules having normal morphology were observed in Group-C while the number of atrophic and degenerative seminiferous tubules also decreased significantly. \nConclusion: Resveratrol is a potent protective agent with promising results in attenuating cisplatin-induced oxidative stress and eventually testicular toxicity.","PeriodicalId":33701,"journal":{"name":"Journal of Islamabad Medical and Dental College","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Islamabad Medical and Dental College","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35787/jimdc.v11i1.716","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

Abstract

Background and Objective: Resveratrol is a poly-hydroxyphenol plant toxin that alleviates oxidative stress by increasing endogenous antioxidant levels and prevents tissue damage. The study aimed to investigate the anti-oxidative role of Resveratrol by histochemical, ultrastructural, and biochemical methods in Cisplatin-induced testicular toxicity in Wistar Albino rats. Material and Methods: Quasi-experimental study was conducted at the Department of Pharmacology, Anatomy and Postgraduate Laboratory of ISRA University Hyderabad from October 2020 to March 2021. Twenty-four male, normal adult Wistar albino rats were recruited and distributed equally into; Group-A (Control), Group-B (Experimental group or Cisplatin group), Group-C (Experimental group or Cisplatin + Resveratrol combination group). Pre and post-experimental body weight, analysis of oxidative markers, semen parameters, and histomorphology were carried out in all three groups. SPSS version 24.0 was used for the analysis of Data. Results: A statistically significant decline in the body weight and testicular weight in group B and C respectively (p<0.05). While reduction in sperm count, motility and viability was observed in Group-B compared with Group-C (p<0.05). Oxidative markers were also significantly depleted in Group-B in comparison to Group-C (p<0.05). Evident changes were observed in the testicular histology of Group-B compared with Group-C (p<0.05). Irregular, regressive, and atrophic seminiferous tubules were seen in Group-B. Most seminiferous tubules having normal morphology were observed in Group-C while the number of atrophic and degenerative seminiferous tubules also decreased significantly. Conclusion: Resveratrol is a potent protective agent with promising results in attenuating cisplatin-induced oxidative stress and eventually testicular toxicity.
白藜芦醇对雄性成年大鼠顺铂睾丸毒性的影响
背景与目的:白藜芦醇是一种多羟基酚植物毒素,通过增加内源性抗氧化剂水平来缓解氧化应激,防止组织损伤。本研究旨在通过组织化学、超微结构和生化等方法探讨白藜芦醇在顺铂致Wistar白化大鼠睾丸毒性中的抗氧化作用。材料与方法:准实验研究于2020年10月至2021年3月在海德拉巴ISRA大学药理学、解剖学和研究生实验室进行。选取24只正常成年雄性Wistar白化大鼠,平均分布于;a组(对照组)、b组(实验组或顺铂组)、c组(实验组或顺铂+白藜芦醇联合组)。对三组大鼠进行实验前和实验后的体重、氧化标志物、精液参数和组织形态学分析。采用SPSS 24.0版本进行数据分析。结果:B、C组大鼠体重、睾丸重量下降,差异均有统计学意义(p<0.05)。与c组相比,b组精子数量、活力和活力均降低(p<0.05)。与c组相比,b组氧化标志物明显减少(p<0.05)。与c组比较,b组睾丸组织学变化明显(p<0.05)。b组精管不规则、退变、萎缩。c组大多数精管形态正常,而萎缩和退行性精管数量也明显减少。结论:白藜芦醇是一种有效的保护剂,在减轻顺铂诱导的氧化应激和最终睾丸毒性方面具有良好的效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
35
审稿时长
16 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信