SARS-CoV-2 infection causes hyperglycaemia in cats

Yufei Zhang, Jindong Gao, Kun Huang, Ya Zhao, Xianfeng Hui, Ting Wang, Changmin Hu, Xiaomei Sun, Ying Yang, Chao Wu, Xi Chen, Zhong Zou, Lianzhong Zhao, M. Jin
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引用次数: 1

Abstract

Abstract Isolated reports of new-onset diabetes in patients with COVID-19 have led researchers to hypothesise that SARS-CoV-2 infects the human exocrine and endocrine pancreatic cells ex vivo and in vivo. However, existing research lacks experimental evidence indicating that SARS-CoV-2 can infect pancreatic tissue. Here, we found that cats infected with a high dose of SARS-CoV-2 exhibited hyperglycaemia. We also detected SARS-CoV-2 RNA in the pancreatic tissues of these cats, and immunohistochemical staining revealed the presence of SARS-CoV-2 nucleocapsid protein (NP) in the islet cells. SARS-CoV-2 NP and Spike proteins were primarily detected in Glu+ cells, and most Glu+ cells expressed ACE2. Additionally, immune protection experiments conducted on cats showed that the blood glucose levels of immunised cats did not increase post-challenge. Our data indicate the cat pancreas as a SARS-CoV-2 target and suggest that the infection of Glu+ cells could contribute to the metabolic dysregulation observed in SARS-CoV-2-infected cats.
SARS-CoV-2感染会导致猫出现高血糖
孤立的COVID-19患者新发糖尿病的报道使研究人员假设SARS-CoV-2在体外和体内感染人类外分泌和内分泌胰腺细胞。然而,现有研究缺乏实验证据表明SARS-CoV-2可以感染胰腺组织。在这里,我们发现感染了高剂量SARS-CoV-2的猫表现出高血糖。我们还在这些猫的胰腺组织中检测到SARS-CoV-2 RNA,免疫组织化学染色显示胰岛细胞中存在SARS-CoV-2核衣壳蛋白(NP)。SARS-CoV-2 NP和Spike蛋白主要在Glu+细胞中检测到,大多数Glu+细胞表达ACE2。此外,在猫身上进行的免疫保护实验表明,免疫猫的血糖水平在攻击后没有增加。我们的数据表明猫胰腺是SARS-CoV-2的靶点,并表明Glu+细胞的感染可能导致SARS-CoV-2感染猫的代谢失调。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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