Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)

IF 1 4区生物学 Q3 PLANT SCIENCES

Indian Journal of Genetics and Plant Breeding Pub Date : 2020-09-22 DOI:10.31742/IJGPB.80.3.18

R. S. Badere, Pallavi K. Rinkey

引用次数: 0

Abstract

The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.

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诱变剂处理对鸡冠花(Celosia cristate L.)茎尖外植体再生效率的影响

将甲烷磺酸乙酯(EMS)诱变苗和伽马射线(GR)诱变苗的茎尖外植体在添加NAA和BAP的MS培养基上培养5代，扩增突变部分。诱变剂降低了外植体的再生效率，影响了外植体对植物生长调节剂诱导多芽的依赖。经gr处理的12d龄苗茎尖诱导的NAA浓度为0.5µM + BAP浓度为6.6µM;8.8µM BAP对ems处理幼苗诱导苗的影响。本研究证实诱变剂影响外植体的再生过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Genetics and Plant Breeding PLANT SCIENCES-

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.