Aerobic Bacterial Community of the Rice Striped Stem Borer: A Step towards Finding Bacterial Candidates for Paratransgenesis and RNAi Control

Abbas Heydari, M. Oshaghi, A. Nazari, M. Shayeghi, E. Sanatgar, N. Choubdar, M. Koosha, F. Karimian
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Abstract

It is recognized that insects have close associations with a wide variety of microorganisms, which play a vital role in the insect's ecology and evolution. The rice striped stem borer, Chilo suppressalis, has economic importance at the global level. With the development of insecticide resistance, it is widely recognized that control of this pest is likely to need new tools that are not available today. Here, we focus on the aerobic bacterial community of the pest, to seek candidates for paratransgenesis or RNAi biocontrol of C. suppressalis. Culture-dependent PCR-direct sequencing was used to characterize the midgut bacterial communities of C.suppressalis at different life stages, collected in northern Iran, both from rice plants and from weeds on which the insect Int J Plant Anim Environ Sci 2021; 11 (3): 485-502 DOI: 10.26502/ijpaes.202117 International Journal of Plant, Animal and Environmental Sciences Vol. 11 No. 3 – September 2021 486 feeds. Our results show that the predominant genera of the Firmicutes and Proteobacteria phyla were Bacillus, Enterobacter and Klebsiella in all life stages, and they displayed differences in abundance. The high levels of B. subtilis and Enterobacter found in C. suppressalis suggests their potential utility for paratransgenesis and RNAi approaches to control this pest.
水稻条纹茎螟虫的需氧细菌群落:寻找副嬗变和RNAi控制的候选细菌的一步
人们认识到昆虫与各种各样的微生物有着密切的联系,这些微生物在昆虫的生态和进化中起着至关重要的作用。水稻条纹螟虫(Chilo suppressalis)在全球具有重要的经济意义。随着杀虫剂耐药性的发展,人们普遍认识到,控制这种害虫可能需要目前无法获得的新工具。在这里,我们专注于害虫的好氧细菌群落,寻找候选的副突变或RNAi生物防治抑孢梭菌。培养依赖pcr -直接测序用于表征在伊朗北部收集的不同生命阶段的C.suppressalis中肠细菌群落,这些细菌来自水稻植物和杂草,昆虫Int J Plant动物环境科学2021;11 (3): 485-502 DOI: 10.26502/ijpaes.202117国际植物,动物和环境科学杂志第11卷第3期- 2021年9月486饲料。结果表明,厚壁菌门和变形菌门在所有生命阶段的优势属为芽孢杆菌、肠杆菌和克雷伯菌,且丰度存在差异。在抑孢梭菌中发现的高水平枯草芽孢杆菌和肠杆菌表明它们在副嬗变和RNAi方法控制该害虫方面的潜在效用。
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