Chemical Constituents of an Iranian Grown Capsicum annuum and their Cytotoxic Activities Evaluation

Abstract

A sample of cayenne pepper ,Capsicum annuum –(Family Solanaceae) was collected from a region in Hamadan, west Iran and extracted from EtOH. GC-MS analysis showed at least 8 compounds out of which 7 compounds were isolated and characterized using 1 H NMR analysis. Cytotoxic effects of the 7 isolated and characterized compounds along with the extract were evaluated against Caco-2 cell lines using MTT assay. The IC 50 values for ethyl palmitate (1), linolelaidic acid ethyl ester (2), ethyl stearate (3), ethyl iso-allocholate (4), 2-(((2-ethylhexyl)oxy)carbonyl) benzoic acid (5), capsaicin (6), dihydrocapsaicin (7) and the extract mixture were determined in μ M (111, 112, 118, 40, 130, 91, 115, and 110, respectively). The data indicated higher cytotoxic effects of (4) and (6) suggested as potential compounds against Caco-2 cell lines.