Protein Kinase C Inhibitors Prevent Cultured Human Endothelial Cell Stress Fiber Formation, but not Heightened Endocytosis

Endothelium-journal of Endothelial Cell Research Pub Date : 1996-01-01 DOI:10.3109/10623329609024697

V. Patel, Jamie W. Meyer, David K. Johnson, R. Abdul-karim, L. M. Ziegler, L. Kauffman, K. Schillinger, L. Lemanski, J. Holland

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引用次数: 5

Abstract

In order to study the signal transduction mechanism of endothelial perturbation, the effects of phorbol 12-myristate 13-acetate (PMA) and phorbol 12,13-dibutyrate (PDBu), both protein kinase C (PKC) activators, on cultured human endothelial cell (EC) hydrogen peroxide (H2O2) generation, endocytotic activity, and cytoskeletal structure have been investigated. EC were incubated with 1-100 nM PMA, or PDBu, and cellular H2O2 generation and endocytotic activity measured. PMA and PDBu exposure caused dose-dependent rises in EC H2O2 production. Likewise, EC incubated with PMA and PDBu had dose-related endocytosis increases. Cytoskeletal inspection of 10 nM PMA-perturbed EC revealed structural remodeling with stress fiber formation. Similar cellular functional changes occur in EC exposed to high low-density lipoprotein (LDL) concentrations. Protein kinase C (PKC) inhibition with 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H-7) prevented cytoskeletal remodeling in PMA-stimulated EC. In differenc...

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蛋白激酶C抑制剂阻止培养的人内皮细胞应激纤维的形成，但不增加内吞作用

为了研究内皮扰动的信号转导机制，研究了蛋白激酶C (PKC)激活剂phorbol 12-肉豆酸13-乙酸酯(PMA)和phorbol 12,13-二丁酸酯(PDBu)对培养的人内皮细胞(EC)过氧化氢(H2O2)生成、内吞活性和细胞骨架结构的影响。EC与1-100 nM PMA或PDBu孵育，测定细胞H2O2生成和内吞活性。PMA和PDBu暴露引起EC H2O2产量的剂量依赖性上升。同样，与PMA和PDBu孵育的EC具有剂量相关的内吞作用增加。10 nM pma扰动EC的细胞骨架检查显示结构重构和应力纤维形成。类似的细胞功能变化发生在暴露于高低密度脂蛋白(LDL)浓度的EC中。1-(5-异喹啉磺酰基)-2-甲基哌嗪二盐酸盐(H-7)抑制蛋白激酶C (PKC)可阻止pma刺激的EC细胞骨架重塑。在differenc…

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Endothelium-journal of Endothelial Cell Research

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