The iodination of tyrosine by myeloperoxidase and beef thyroids. The possible involvement of free radicals

Cecil C. Yip, Linda D. Hadley
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引用次数: 18

Abstract

  • 1.

    1. The oxidation of sulfite, measured by the uptake of oxygen, was employed to detect the formation of free radicals in the iodination of tyrosine by myeloperoxidase in the presence of added H2O2.

  • 2.

    2. The uptake of oxygen was dependent on sulfite, tyrosine and myeloperoxidase. Tyrosine could be replaced by monoiodotyrosine, diiodotyrosine, 4-hydroxy-3,5-diiodophenylacetic acid or 4-hydroxy-3,5-diiodobenzaldehyde but not by thyroglobulin, phenylacetic acid, phenylalanine or iodide. Iodide was inhibitory.

  • 3.

    3. The oxidation of sulfite under these conditions was affected by pH and had an optimal range between pH 5 and 6.5.

  • 4.

    4. Sulfite and bisulfite inhibited completely the iodination of tyrosine and of thyroglobulin by myeloperoxidase in the presence of added H2O2. Sulfate was without effect.

  • 5.

    5. The iodination of tyrosine by myeloperoxidase in the presence of added H2O2 showed the same pH optimum as the oxidation of sulfite.

  • 6.

    6. A concentrated solubilized preparation of beef thyroid tissues, which catalyzed the peroxidation of o-dianisidine and the iodination of tyrosine, could not substitute effectively for myeloperoxidase in the oxidation of sulfite. However, the iodination of tyrosine by this enzyme preparation was inhibited by sulfite and bisulfite.

  • 7.

    7. These results are discussed in relation to a free radical reaction mechanism involved in the synthesis of iodotyrosines by myeloperoxidase and by the beef thyroid preparation.

髓过氧化物酶和牛肉甲状腺对酪氨酸的碘化作用。自由基的可能参与
1.1. 亚硫酸盐的氧化用摄氧量来测定,在加入H2O2.2.2的情况下,髓过氧化物酶对酪氨酸碘化反应中自由基的形成。氧的摄取依赖于亚硫酸盐、酪氨酸和髓过氧化物酶。酪氨酸可被单碘酪氨酸、二碘酪氨酸、4-羟基-3,5-二碘苯乙酸或4-羟基-3,5-二碘苯甲醛替代,但不能被甲状腺球蛋白、苯乙酸、苯丙氨酸或碘化物替代。碘化物具有抑制作用。在此条件下,亚硫酸盐的氧化受pH值的影响,pH值在5 ~ 6.5.4.4之间为最佳氧化范围。亚硫酸盐和亚硫酸氢盐完全抑制了髓过氧化物酶对酪氨酸和甲状腺球蛋白的碘化作用。硫酸钠无效果。在加入H2O2的条件下,髓过氧化物酶对酪氨酸的碘化反应pH值与亚硫酸盐的氧化反应pH值相同。牛甲状腺组织浓溶制剂虽然能催化邻二苯胺的过氧化和酪氨酸的碘化,但不能有效替代髓过氧化物酶对亚硫酸盐的氧化作用。然而,该酶制剂对酪氨酸的碘化作用受到亚硫酸盐和亚硫酸氢盐的抑制。这些结果讨论了与自由基反应机制有关的髓过氧化物酶和牛肉甲状腺制剂中碘酪氨酸的合成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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