Hepatoprotective activity of ethyl acetate fraction from Lygodium microphyllum leaves in CCl4 induced damage rats

Abstract

Lygodium microphyllum is one of the plants that have the potential as an agent hepatoprotective because known of these plants have been used as the chinese traditional medicine. They use it to treat hepatitis diseases with drink a stew of the plant. The purpose of this study is to study the effects of a ethyl acetate fraction as hepatoprotective from L. microphyllum leaves in CCl4 induced damaged rats. This study uses animals of about 36 rats divided into six groups for 14 days. On the 15th-day, animal testing sacrificed and extracted the blood and surely to know the activity of the SGOT and SGPT and hold the histological structure of the liver to know the liver damage. The result showed the idea of a fraction of ethyl acetate leaves L. microphyllum may lower levels of SGOT significantly (p<0,05) but cannot be lower the levels SGPT significantly. The highest SGPT activity indicated by the ethyl acetate leaves L. microphyllum 200 doses of mg/kg b.w that measure being 202.50±88.459 U/L compared with the negative of 2362.50±1213.592 U/L. Examination histopathology scoring liver damage done by counting the number of cells from normal hepatocytes and suffered from damage (degeneration and necrosis ). The administration of L. microphyllum leaf ethyl acetate fraction did not affect mice weight. Faction doses of ethyl acetate leave L.microphyllum 200 mg/kg b.w show that there was a protective effect against the hearts. Based on the results, it can be concluded that ethyl acetate faction leaves L. microphyllum having the effect of hepatoprotective against CCL4 induced damage rats.