Evaluation of Cellular miR-122 Expression in Association with the Presence of Varicella-Zoster Virus among Central Nervous System Tumors

IF 0.4 Q4 ONCOLOGY
Aida Abbasi, Javid Sadri Nahand, Mohsen Moghoofei, Maryam Esghaei, D. Javanmard, M. H. Karbalaie Niya, Farzin Sadeghi, F. Bokharaei-Salim, M. Karimzadeh, A. Khodayari, H. Keyvani
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引用次数: 0

Abstract

Background: Brain tumors are all primary central nervous system (CNS) tumors with unclear etiologies and viral infections, especially human herpesviruses, which have emerged as a hot topic for comprehensive research. Objectives: The present study aimed at assessing the molecular epidemiology of varicella-zoster virus (VZV) and its association with microRNA 122 (miR-122) expression in CNS tumor samples. Methods: Fresh frozen tissue samples were collected from 60 CNS tumor patients and 45 healthy controls. A nested PCR assay was performed to detect the VZV-DNA. Subsequently, the expression level of miR-122 was evaluated in the CNS tumor tissue samples of patients and the brain tissue samples were obtained from healthy controls, using a real-time PCR assay. Results: Of 60 patients with CNS tumors, 29 were men and 31 were women. VZV-DNA was detected in 13.3% of the CNS tumor tissue specimens. There was no statistically significant association between the presence of VZV-DNA and different types of CNS tumors (P > 0.05). Furthermore, the expression level of miR-122 was significantly downregulated in the CNS tumor tissue samples obtained from the patients compared with those of the healthy controls (P < 0.05). Additionally, the expression level of miR-122 was significantly lower in the VZV-positive tumor samples as compared with those of the VZV-negative tumor samples and the healthy controls. Conclusions: Although VZV plays no direct role in the development of CNS tumors, the virus may affect the biology of CNS tumors by decreasing the expression levels of miR-122, which consequently leads to an increased risk of malignancy. However, the experimental data are not conclusive enough; so, further investigations are needed.
中枢神经系统肿瘤细胞miR-122表达与水痘带状疱疹病毒存在相关性的评价
背景:脑肿瘤均为原发性中枢神经系统(CNS)肿瘤,病因不明,常受病毒感染,尤其是人疱疹病毒感染,已成为综合性研究的热点。目的:本研究旨在评估水痘-带状疱疹病毒(VZV)的分子流行病学及其与中枢神经系统肿瘤样本中microRNA 122 (miR-122)表达的关系。方法:采集60例中枢神经系统肿瘤患者和45例健康对照者的新鲜冷冻组织标本。采用巢式PCR法检测VZV-DNA。随后,使用实时PCR检测miR-122在患者中枢神经系统肿瘤组织样本和健康对照脑组织样本中的表达水平。结果:60例中枢神经系统肿瘤中,男性29例,女性31例。13.3%的CNS肿瘤组织标本中检测到VZV-DNA。VZV-DNA的存在与不同类型中枢神经系统肿瘤之间的相关性无统计学意义(P < 0.05)。此外,与健康对照组相比,患者CNS肿瘤组织样本中miR-122的表达水平显著下调(P < 0.05)。此外,miR-122在vzv阳性肿瘤样本中的表达水平明显低于vzv阴性肿瘤样本和健康对照。结论:虽然VZV在中枢神经系统肿瘤的发生发展中没有直接作用,但该病毒可能通过降低miR-122的表达水平来影响中枢神经系统肿瘤的生物学特性,从而导致恶性肿瘤的风险增加。然而,实验数据还不够确凿;因此,需要进一步的调查。
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来源期刊
CiteScore
1.10
自引率
0.00%
发文量
67
期刊介绍: International Journal of Cancer Management (IJCM) publishes peer-reviewed original studies and reviews on cancer etiology, epidemiology and risk factors, novel approach to cancer management including prevention, diagnosis, surgery, radiotherapy, medical oncology, and issues regarding cancer survivorship and palliative care. The scope spans the spectrum of cancer research from the laboratory to the clinic, with special emphasis on translational cancer research that bridge the laboratory and clinic. We also consider original case reports that expand clinical cancer knowledge and convey important best practice messages.
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