Evaluation of Neuroprotective Property of Madhunashini Vati in Diabetic Rat

Abstract

The present study was designed to evaluate the Neuroprotection property of Madhunashini Vati in Diabetic Rat. Eight groups of rat and each group comprising of six animals were employed in the present study. During the experiment different doses of Madhunashini Vati (125, 250, 500 mg/kg) was administered. Insulin (4 IU/kg body weight s.c) was administered daily before the global ischemia in diabetic rats. Rats were treated with Streptozotocin (STZ) 65 mg/kg i.p for the induction of Diabetes for 14 days. Rats were anaesthetized by using chloral hydrate (400 mg/kg, i.p) and Global cerebral ischemia was induced by occluding the carotid arteries, reperfusion was allowed for 24 hours. At the end of study, total Serum Glucose level, Serum Triglyceride level, Thiobarbituric Acid Reactive Substances(TBARS) and Cerebral Infarct size was estimated for the Neuroprotection property. Administration of Madhunashini Vati, showed reduction in the serum glucose level, serum triglycerides level, cerebral infarct size and increases in body weight. In the present study we observed that the madhunashini vati significantly improved cerebral ischemia. Results indicate that Madhunashini Vati (MV) is a good neuroprotective and could be used as a therapeutic treatment in brain stroke. And it is likely to be responsible for the free radical scavenging activity and rejuvenate the beta cells observed.