Effects of inhibitors and activators on the activity of two diaphorases from Drosophila virilis

Abstract

The effect of some inhibitors and activators of mammalian DT-diaphorase on diaphorase-1 (DIA-1) and diaphorase-2′ (DIA-2′) purified from Drosophila virilis was studied. The inhibitors and activators changed the activity of these diaphorases in a different way, revealing a similarity between mammalian DT-diaphorase and D. virilis DIA-1 on the one hand and on the other between the D. virilis DIA-1 and the diaphorase purified from Bombyx mori eggs. These effects also confirm the independent genetic control of DIA-1 and DIA-2′ in D. virilis and make possible the differentiation of these diaphorase activities in crude enzyme extracts.