Effect of Tragacanth on Some Morpho-physiological Characteristics Related to Black cumin (Nigella sativa L.) Yield Under Drought Stress Conditions

Abstract

Introduction In fact, drought is stress that restricts the plant photosynthesis and causing changes in the chlorophyll content and damage to photosynthetic structures. One of the important reasons that environmental stresses such as drought reduce the growth and photosynthesis ability of the plant is a disturbance in the balance between production and removal of free oxygen radicals. Transpiration is a necessary process for photosynthesis and growth of plants but depending on the conditions may be harmful in some cases, so that the use of anti-transpirant can be one of the most effective methods for reducing the amount of water lost through transpiration and effective way to reduce the effects of drought stress on plants and adjustment the reduction of the yield due to water deficiency in arid and semi-arid regions. Climate change created in the world and the intensification of stresses caused by it, especially drought stress in arid and semi-arid regions such as Iran, make it difficult to achieve this goal. Therefore, finding strategies that can reduce the effects of water shortages on plant growth and yield and improve growth and yield can be very important. The aim of the present study was to investigate the effect of irrigation regimes and application of different concentrations of tragacanth (naturally dried exudate from some Astragalus species) on black cumin plant. Materials and Methods This research was carried out in a factorial experiment based on completely randomized design with three replications in a greenhouse of the Agriculture College of Kurdistan University in 2018. The experimental factors were including irrigation at three levels of 100% (full irrigation), 70% (mild drought stress), and 40% (severe drought stress) of field capacity of soil and spraying with tragacanth extract at six concentrations of 0, 1.25, 2.5, 5, 7.5, and 10 g/L. Spraying of this material was done using a back sprayer (Shark model) with a constant pressure of 2.4 bar and a volume of 250 liters of water per hectare. Before the data were analyzed, their normality test was performed using the Mini Tab software. After ensuring the normality of data, analysis of variance was performed using SAS ver. 9.3. LSD (Least significant difference) was used to compare the mean of treatments. The graphs are drawn using Excel software. Results and Discussion The results showed that increased drought stress intensity (irrigation reduction) led to the reduced leaf relative water content, Total chlorophyll content, efficiency of photosystem II, plant hight, number of capsules per plant, mean number of seeds per plant, biological yield and grain yield. The positive effects of tragacanth consumption on reducing and modifying the effects of drought stress on different levels of irrigation and different concentrations of tragacanth were different. In the present study, under full irrigation conditions, lower concentrations of tragacanth were useful, while in drought stress conditions, higher concentrations of tragacanth (except 10 g/L) were useful. In full irrigation, the concentration of 1.25 g/L was positive for all studied traits. In mild drought stress, the use of higher concentrations of tragacanth up to 5 g/L had the best effect and more concentrations resulted in a reverse effect on studied traits. In severe drought stress, the use of more concentrations of tragacanth extract was beneficial and improved the studied traits up to 7.5 g/L, but 10 g/L had a negative effect on these traits. Considering the effect of proper concentration of this material on the improvement of measured traits and increasing the grain yield compared to non-application of tragacanth under severe and moderate drought stress conditions by 11.6% and 28.2%, respectively. Conclusion The results of this study indicated the different effects of various concentrations of tragacanth material in different levels of irrigation on studied traits of black cumin. Therefore, it can be concluded that the application of different concentrations of tragacanth gum was completely dependent on the plant's water status. So that, using higher concentrations of tragacanth gum in drought stress conditions had a more positive effect on the plant, and vice versa, using a lower concentration of this material was useful in full irrigation. The effect of tragacanth gum on reducing and modifying the effects of drought stress in different plants requires further studies and extensive research. Tragacanth gum can be introduced as a new anti-transpirant agent with natural origin and its application can be useful and recommended in areas exposed to drought stress.