Development of A Rapid Microtiterplate Based Colorimetric Method for Estimation of Non-esterified Fatty Acids in Bovine Plasma

Abstract

Non-esterified fatty acid (NEFA) is one of the most important blood metabolites contributing significantly during body growth, cyclicity, pregnancy and lactation, and it plays more vital role particularly during transition phase of the biological processes like pregnancy to lactation transition period. Determination of blood concentrations of NEFA is, therefore, important. The measurement technique or assay for measurement of blood NEFA should be simple, reliable, accurate, rapid and cheap. Here, the authors developed a sensitive and fast microtiterplate colorimetry-based method for estimation of NEFA in bovine plasma. The method is simple to perform and gives accurate, precise results in 100 µL samples. The developed method requires at least five times less quantity of plasma and reagents than that of already existing methods. Furthermore, ability of the method for taking reading of all the samples together on microtiterplate in a reader enable us to save a lot of time. Hence, the developed method may be adopted for estimation of NEFA in bovine plasma. Keywords: NEFA, bovine, microtiterplate, plasma, assay Cite this Article Mondal M, Baruah KK. Development of A Rapid Microtiterplate Based Colorimetric Method for Estimation of Non-Esterified Fatty Acids in Bovine Plasma. Research & Reviews: Journal of Veterinary Science and Technology . 2015; 4(3): 29–34p.