Laser Light Scattering-Based Label-Free Cytometry

Abstract

A label-free cytometry technique based on laser light scattering is presented as an alternative to conventional flow cytometry using fluorescent or magnetic markers. Single-cell light scattering patterns are used as fingerprints for cell identification. Blood cells and neuroblastoma cells have been used as experimental case studies. Various methods including machine learning have been deployed for the analysis of the laser light scattering patterns. Potential applications of this label-free technique will be discussed.