MRI Features and Significance of Serum miRNAs and Inflammatory Cytokines in Patients with Temporal Lobe Epilepsy

IF 0.4 4区化学 Q4 CHEMISTRY, PHYSICAL

Concepts in Magnetic Resonance Part A Pub Date : 2022-10-04 DOI:10.1155/2022/3401838

Nengyun Yao, Yanmei She, Songlin Tang, Huafei Liu, Fang Liu

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Abstract

This study was aimed to investigate the changes of brain MRI features and serum biological parameters in patients with TLE. 30 patients with unilateral TLE confirmed by surgical pathology were selected as study subjects, and 30 subjects without a history of epilepsy who underwent health examinations during the same period were selected as controls. The brain MRI features of the patients were explored and the T2 relaxation time (HCT2) indexes of the bilateral hippocampus were extracted. The differences in levels of peripheral blood T lymphocyte subsets, inflammatory cytokines, and miRNAs were measured. The results showed that the hippocampal volume of TLE patients was significantly reduced, and the HCT2 value of the hippocampus was greater than that of the control group ( P < 0.05 ). CD3+ (77.9 ± 4.4)%, CD4+ (45.6 ± 2.2)%, CD8+ (22.1 ± 1.9)%, and CD3+/CD8+ (2.24 ± 0.22) in peripheral blood T lymphocyte subsets of epileptic patients, compared with control group, CD3+, CD4+, and CD4+/CD8+ levels were significantly increased and CD8+ concentration was significantly decreased in epileptic patients ( P < 0.05 ); inflammatory cytokines TNF-α was (2.63 ± 0.26) pg/mL, IL-1β was (4.61 ± 0.57) pg/mL, IL-2 was (1.59 ± 0.21) pg/mL, IL-6 was (2.28 ± 0.19) pg/mL, and ICAM-1 was (1.89 ± 0.30) pg/mL in peripheral blood of epileptic patients, which was significantly increased compared with control group, while IL-10 was significantly decreased in epileptic patients ( P < 0.05 ); miR-146a was (2.14 ± 0.28) and miR-210 was (1.89 ± 0.31), miR-221 (2.44 ± 0.35), miR-34a (0.59 ± 0.14), miR-135b (10.17 ± 0.16), miR-33 (0.26 ± 0.09) in peripheral blood miRNA levels of epileptic patients, and miR-146a, miR-210, miR-221, and miR-34a levels of epileptic patients were significantly increased compared with control group, while MiR-135b and miR-33 levels of epileptic patients were significantly reduced ( P < 0.05 ). In summary, patients with TLE have hippocampal lesions, which may be related to peripheral blood T lymphocyte subsets imbalance, chronic inflammatory response, and abnormal expression of miRNAs.

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颞叶癫痫患者血清mirna和炎性细胞因子的MRI特征及意义

本研究旨在探讨TLE患者的脑MRI特征和血清生物学参数的变化。选取经手术病理证实的单侧TLE患者30例作为研究对象，选取同期接受健康检查的无癫痫史患者30例作为对照。探讨患者的脑部MRI特征，提取双侧海马T2松弛时间(HCT2)指数。测量外周血T淋巴细胞亚群、炎症细胞因子和mirna水平的差异。结果显示，TLE患者海马体积明显减小，海马HCT2值高于对照组(P < 0.05)。癫痫患者外周血T淋巴细胞亚群CD3+(77.9±4.4)%、CD4+(45.6±2.2)%、CD8+(22.1±1.9)%、CD3+/CD8+(2.24±0.22)水平与对照组比较，CD3+、CD4+、CD4+/CD8+水平显著升高，CD8+浓度显著降低(P < 0.05);癫痫患者外周血炎症因子TNF-α为(2.63±0.26)pg/mL, IL-1β为(4.61±0.57)pg/mL, IL-2为(1.59±0.21)pg/mL, IL-6为(2.28±0.19)pg/mL, ICAM-1为(1.89±0.30)pg/mL，均较对照组显著升高，IL-10显著降低(P < 0.05);癫痫患者外周血miRNA水平miR-146a为(2.14±0.28)，miR-210为(1.89±0.31)，miR-221(2.44±0.35)，miR-34a(0.59±0.14)，miR-135b(10.17±0.16)，miR-33(0.26±0.09)，与对照组相比，癫痫患者外周血miR-146a、miR-210、miR-221、miR-34a水平显著升高，miR-135b、miR-33水平显著降低(P < 0.05)。综上所述，TLE患者存在海马病变，可能与外周血T淋巴细胞亚群失衡、慢性炎症反应、mirna表达异常有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Concepts in Magnetic Resonance Part A 物理-光谱学

CiteScore

0.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Concepts in Magnetic Resonance Part A brings together clinicians, chemists, and physicists involved in the application of magnetic resonance techniques. The journal welcomes contributions predominantly from the fields of magnetic resonance imaging (MRI), nuclear magnetic resonance (NMR), and electron paramagnetic resonance (EPR), but also encourages submissions relating to less common magnetic resonance imaging and analytical methods. Contributors come from academic, governmental, and clinical communities, to disseminate the latest important experimental results from medical, non-medical, and analytical magnetic resonance methods, as well as related computational and theoretical advances. Subject areas include (but are by no means limited to): -Fundamental advances in the understanding of magnetic resonance -Experimental results from magnetic resonance imaging (including MRI and its specialized applications) -Experimental results from magnetic resonance spectroscopy (including NMR, EPR, and their specialized applications) -Computational and theoretical support and prediction for experimental results -Focused reviews providing commentary and discussion on recent results and developments in topical areas of investigation -Reviews of magnetic resonance approaches with a tutorial or educational approach