Production and partial characterization of neutral protease by an indigenously isolated strain of Aspergillus tubingensis NIICC-08155

Abstract

An indigenously isolated strain of Aspergillus tubingensis NIICC-08155 from soil of teak dominated vegetation of kusmi forest at Gorakhpur was subjected to neutral protease production and its partial characterization. The maximum production of neutral protease i.e. 68.50 U/ml was attained after 96h of incubation. The crude preparation of protease showed reasonable activity at temperature range of 40 to 60 °C with maximum activity at 40 oC and had a maximum enzyme activity at pH 6.4. The Km value of the enzyme was found to be 45.0 mg/ml and the molecular weight as determined by SDS-PAGE was approximately 45 kDa. The enzyme was completely inhibited by DTT and EDTA.