Cytotoxicity and Antifungal Activities of Root Bark of Calotropis gigantea

Stamford Journal of Pharmaceutical Sciences Pub Date : 2010-08-12 DOI:10.3329/SJPS.V2I2.2187

M. R. Habib, M. Alam, M. Haque, Farjana Nikkon, M. R. Karim

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引用次数: 3

Abstract

In this study, methanol extract from the root bark of Calotropis gigantea L. and its petroleum ether (40°C- 60°C), chloroform and ethyl acetate soluble fractions were tested for their cytotoxic activity against brine shrimp nauplii ( Artemia salina , Leach) and for antifungal activity against Aspergillus flavus , Aspergillus niger , Penicillium sp and Trichoderma harzianum . Thin layer chromatography (TLC) screening showed that methanol extract and its different fractions contained different type compounds such as steroid, terpene, glycoside, heterocyclic and flavonoid. In brine shrimp lethality bioassay, it was found that chloroform fraction was highly cytotoxic (LD 50 14.72 μg/ml) among the tested samples. Though methanol extract and ethyl acetate fraction have no activity against all the tested fungi but petroleum ether and chloroform fractions showed potent activity against Aspergillus niger , Penicillium sp, Trichoderma harzianum and Aspergillus niger , Trichoderma harzianum , respectively, in antifungal activity test. Key words: Calotropis gigantea ; Methanol extract; Antifungal activity; Cytotoxicty. DOI: 10.3329/sjps.v2i2.2187 Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 38-41

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巨角牛蒡根皮的细胞毒性及抗真菌活性研究

本研究以Calotropis gigantea L.根皮甲醇提取物及其石油醚(40°C ~ 60°C)、氯仿和乙酸乙酯可溶性组分为研究对象，测定了其对盐渍对虾(Artemia salina, Leach)的细胞毒活性，以及对黄曲霉、黑曲霉、青霉和哈茨木霉的抑菌活性。薄层色谱(TLC)筛选表明，甲醇提取物及其不同馏分中含有甾体、萜烯、糖苷、杂环和类黄酮等不同类型的化合物。在卤虾致死性生物测定中，氯仿部分具有较高的细胞毒性(ld50为14.72 μg/ml)。甲醇提取物和乙酸乙酯部位对所有真菌均无抑制作用，而石油醚和氯仿部位对黑曲霉、青霉、哈茨木霉和黑曲霉、哈茨木霉分别有抑制作用。关键词:大菱角;甲醇提取;抗真菌活性;Cytotoxicty。DOI: 10.3329/sjps.v2i2.2187斯坦福药物科学杂志Vol.2(2) 2009: 38-41

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Stamford Journal of Pharmaceutical Sciences

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