Conferring the Midas Touch on Integrative Taxonomy: A Nanogold-Oligonucleotide Conjugate-Based Quick Species Identification Tool

IF 0.2 Q4 ECOLOGY
Rahul Kumar, Ajay Kumar Sharma
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引用次数: 0

Abstract

Nanogold or functionalized gold nanoparticles (GNPs) have myriad applications in medical sciences. GNPs are widely used in the area of nanodiagnostics and nanotherapeutics. Applications of GNPs in taxonomic studies have not been studied vis-à-vis its extensive medical applications. GNPs have great potential in the area of integrative taxonomy. We have realized that GNPs can be used to visually detect animal species based on molecular signatures. In this regard, we have synthesized gold nanoparticles (<20 nm) and have developed a method based on interactions between thiolated DNA oligonucleotides and small-sized GNPs, interactions between DNA oligonucleotides and target DNA molecules, and self-aggregating properties of small-sized GNPs under high salt concentrations leading to a visible change in colour. Exploiting these intermolecular and interparticle interactions under aqueous conditions, in the present work, we have demonstrated the application of our procedure by using a DNA oligonucleotide probe designed against a portion of the mitochondrial genome of the codling moth Cydia pomonella. This method is accurate, quick, and easy to use once devised and can be used as an additional tool along with DNA barcoding. This tool can be used for distinguishing cryptic species, identification of morphovariants of known species, diet analysis, and identification of pest species in quarantine facilities without any need of performing repetitive DNA sequencing. We suggest that designing and selecting a highly specific DNA probe is crucial in increasing the specificity of the procedure. Present work may be considered as an effort to introduce nanotechnology as a new discipline to the extensive field of integrative taxonomy with which disciplines like palaeontology, embryology, anatomy, ethology, ecology, biochemistry, and molecular biology are already associated for a long time.
赋予整合分类的点石成金:基于纳米金寡核苷酸缀合物的快速物种鉴定工具
纳米金或功能化金纳米粒子(GNPs)在医学科学中有着无数的应用。GNPs广泛应用于纳米诊断和纳米治疗领域。GNPs在分类学研究中的应用尚未研究-à-vis其广泛的医学应用。GNPs在综合分类学领域具有很大的应用潜力。我们已经意识到GNPs可以用于基于分子特征的视觉检测动物物种。在这方面,我们已经合成了金纳米粒子(<20 nm),并开发了一种基于硫代DNA寡核苷酸与小尺寸GNPs之间的相互作用,DNA寡核苷酸与靶DNA分子之间的相互作用以及高盐浓度下小尺寸GNPs的自聚集特性导致可见颜色变化的方法。利用这些分子间和粒子间的相互作用在水条件下,在目前的工作中,我们已经证明了我们的程序的应用,通过使用DNA寡核苷酸探针设计针对冷蛾的一部分线粒体基因组的Cydia pomonella。该方法具有准确、快速和易于使用的特点,可以作为DNA条形码的附加工具。该工具可用于区分隐种、鉴定已知物种的形态变异、食性分析和检疫设施中有害生物的鉴定,而无需进行重复的DNA测序。我们建议,设计和选择一个高度特异性的DNA探针是提高该程序的特异性至关重要。在古生物学、胚胎学、解剖学、动物行为学、生态学、生物化学和分子生物学等学科早已与纳米技术联系在一起的广泛的综合分类学领域中,目前的工作可以看作是将纳米技术作为一门新学科引入这一领域的努力。
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来源期刊
自引率
0.00%
发文量
15
期刊介绍: The main aim of the International Journal of Ecology & Development (IJED) is to publish refereed, well-written original research articles, and studies that describe the latest research and developments in ecology and development. It also covers the many potential applications and connections to other areas of Ecological Science, economics and technology such as the use and development of mathematics/statistics in ecology or use and development of economics for ecology & development or inter-disciplinary nature of applications for Ecology & Development. International Journal of Ecology and Development is published three issues in a year in Winter, Summer and Fall.
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