Molecular Detection of Some Capsulargenes of Klebsiella pneumoniaeIsolated from Clinical Samples

Amina A. Raheem, Ghaidaa J. Mohammed
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Abstract

Klebsiella pneumoniae is an opportunistic pathogen that has been implicated as one of commonest cause of hospital and community acquired infections.The capsule of Klebsiella pneumoniae is an important virulence factor, involved in pathogenic mechanisms.So, this study aimed to isolateKlebsiella pneumoniae from different clinical samples from patients in Al-Diwaniyah teaching hospital and determine some virulence factors (capsulegenes)that used for serotyping of isolates. The study extended fromAugust  to November 2020.A total of 31 isolates from 80 different clinical samples identified as Klebsiella pneumoniae by traditional biochemical tests ,Vitek system and 16SrRNA gene.The existenceof three genes from7tested capsulargenes wasdetectedby Polymerase chain reaction.The commonness serotype were k2,k54,k57 where K2 detected in 4 (12.9 %), K54 in 12 (38.7 %),k57 in 4 (12.9).But, the other capsular polysaccaride genes k1,k3,k5,k20not detectedin all isolates of this study.
临床分离肺炎克雷伯菌荚膜原的分子检测
肺炎克雷伯菌是一种机会性病原体,被认为是医院和社区获得性感染的最常见原因之一。肺炎克雷伯菌荚膜是一个重要的毒力因子,参与致病机制。因此,本研究旨在从Al-Diwaniyah教学医院患者的不同临床样本中分离肺炎克雷伯菌,并确定用于分离株血清分型的毒力因子(荚膜基因)。该研究从2020年8月延长至11月。经传统生化试验、Vitek系统和16SrRNA基因鉴定,从80份不同临床样本中分离出31株肺炎克雷伯菌。聚合酶链反应检测了7个荚膜菌中3个基因的存在。常见血清型为k2、k54、k57,其中k2 4例(12.9%),k54 12例(38.7%),k57 4例(12.9%)。而其他荚膜多糖基因k1、k3、k5、k20在本研究的所有分离株中均未检测到。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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