Cyclooxygenase (COX) and 15-Lipoxygenase (15-LOX) Inhibitory Activity and HPTLC Profile of Asplenium Nidus, Diplazium Esculentum, and Drynaria Quercifolia in Bukidnon, Philippines

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
A. Ang, Roxan D. Sabesaje, G. Barbosa, R. Y. D. Cruz, Rainear A. Mendez, Melania M. Enot
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引用次数: 0

Abstract

Diplazium esculentum, Drynaria quercifolia, and Asplenium nidus are among the fern species found in Bukidnon, Philippines which are used as traditional herbal medicines.  The HPTLC profile and the anti-inflammatory properties against cyclooxygenase (COX) and 15-lipoxygenase (15-LOX) of the frond ethanolic extracts of D. esculentum, D. quercifolia, and A. nidus were determined.  The High-Performance Thin Layer Chromatography (HPTLC) profile was obtained using ethyl acetate: formic acid: water (16:2:2) as mobile phase and Natural Products (NP) as derivatizing reagent.  The HPTLC profile of the D. esculentum, D. quercifolia, and A. nidus extracts showed 10 (Rf = 0.02-0.97), 13 (Rf = 0.03-0.90), and 14 (Rf = 0.02-0.99) bands, respectively. The profiles for each fern species may be used as marker for quality evaluation and standardization of herbal formulations containing these plants.  For the anti-inflammatory properties, D. esculentum and D. quercifolia extracts which inhibited more than 50% of the COX enzymes showed significantly higher activity than A. nidus and were considered active against COX-2 and COX-1. D. esculentum, however, gave a selectivity ratio (COX-2/COX-1) of 1.03 making its inhibitory activity selective against COX-2.  The percent 15-LOX inhibitory activity of D. quercifolia (58.62%) is significantly higher than that of A. nidus (38.70%) but statistically comparable to D. esculentum (51.19%).  Among the extracts, D. quercifolia and D. esculentum which inhibited more than 50% of the 15-LOX were considered active. D. esculentum and D. quercifolia can therefore be potential sources of anti-inflammatory lead compounds for future drug development.   
菲律宾Bukidnon地区仙桃、猕猴桃和槲皮草环氧合酶(COX)和15-脂氧合酶(15-LOX)抑制活性及HPTLC谱
在菲律宾Bukidnon发现的蕨类植物中,有双蕨、槲皮树和野蕨,它们被用作传统草药。采用HPTLC谱法测定了三种植物叶面乙醇提取物的环氧合酶(COX)和15-脂氧合酶(15-LOX)抗炎活性。以乙酸乙酯:甲酸:水(16:2:2)为流动相,天然产物(NP)为衍生试剂,建立了高效薄层色谱(HPTLC)图谱。槲皮提取物、槲皮提取物和荆芥提取物的HPTLC谱图分别显示出10个(Rf = 0.02 ~ 0.97)、13个(Rf = 0.03 ~ 0.90)和14个(Rf = 0.02 ~ 0.99)波段。各蕨类植物的谱图可作为含有这些植物的中药制剂质量评价和标准化的标志。对COX-2和COX-1有抑制作用,对50%以上的COX酶有抑制作用,槲皮提取物和金针叶提取物的抗炎活性显著高于金针叶提取物。而对COX-2的选择性比(COX-2/COX-1)为1.03,对COX-2具有选择性抑制活性。槲皮对15-LOX的抑制率为58.62%,显著高于野金针桃(38.70%),与黄金针桃(51.19%)无统计学差异。其中,槲皮和木皮提取物对15-LOX的抑制作用大于50%,具有一定的活性。因此,栓皮栎和槲皮可能是未来药物开发中抗炎先导化合物的潜在来源。
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来源期刊
INDONESIAN JOURNAL OF PHARMACY
INDONESIAN JOURNAL OF PHARMACY PHARMACOLOGY & PHARMACY-
CiteScore
1.20
自引率
0.00%
发文量
38
审稿时长
12 weeks
期刊介绍: The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education. The journal includes various fields of pharmaceuticals sciences such as: -Pharmacology and Toxicology -Pharmacokinetics -Community and Clinical Pharmacy -Pharmaceutical Chemistry -Pharmaceutical Biology -Pharmaceutics -Pharmaceutical Technology -Biopharmaceutics -Pharmaceutical Microbiology and Biotechnology -Alternative medicines.
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