Relationship of IL-6 gene polymorphisms and IL-6 expression level with the burn-induced sepsis susceptibility in Al Diwaniyah

Abstract

Sepsis is one of the most important causes of burn death and sepsis in the hospital after the injury. The present study aimed to investigate the correlation between single nucleotide polymorphism in IL-6 with serum level of IL-6 and MIP-1α expression in the development of burn patients with sepsis and burn patients without sepsis. Enzyme linked immunosorbent assay (ELISA) technique was used to detect serum level of IL-6 and MIP-1α in burn patient with sepsis and burn patient without sepsis. Genomic DNA was extracted from blood for molecular assay to study the correlation between IL-6 -174 G→C , TNF–α -308 G →A gene polymorphism in burn patient with sepsis . Genotyping done by usingTetra Amplification Refractory Mutation System – Polymerase Chain Reaction (T-ARMS-PCR) technique. Immunological assay results of this study showed that the serum level and median (IQR) of IL-6 and MIP-1α were significantly higher in burn patient with sepsis group than in burn patient without sepsis group they were 443.77 pg/ml (471.62) versus 299 pg/ml (295.3) at (P< 0.001) and 148.43 pg/ml (141.9) versus 140.4 pg/ml (144.49) respectively. The present results showed that the IL-6 serum level was significantly higher in the female patients with sepsis compared to the burn patient without sepsis ( P =0.001) . The present study showed that IL-6 was correlated between IL-6 serum and IL-6 genotype in burn patient with sepsis and burn patient without sepsis. Heterozygous GC genotype was significantly higher in correlated between IL-6 serum and IL-6 genotype in burn patient with sepsis and burn patient without sepsis groups (P < 0.001). Also, Homozygous GG genotype was significantly higher in correlated between IL-6 serum and IL-6 genotype in burn patient with sepsis and burn patient without sepsis group (P < 0.002). While, Homozygous CC genotype was not significantly in correlated between IL-6 serum and IL-6 genotype in burn patient with sepsis and burn patient without sepsis control group (P ˃ 0.05) .