Improved clean-up for the determination of lasalocid in 'difficult' food matrices

Abstract

Methodology has been developed for the determination of lasalocid in analytically ‘difficult’ matrices such as processed and spiced foods. The procedure was based on an existing silica-based solid-phase extraction (SPE) clean-up to which was added a novel NH 2 SPE step before HPLC with fluorescence detection. Use of the additional step enabled the determination of lasalocid in matrices such as baby food, meat pies (‘pasties’), etc. Analysis of these matrices was not possible using the standard clean-up on its own. Chromatography showed a massive reduction in the amount of co-extractives and interferences. Validation data were obtained down to the 10-40 μg kg -1 level for a range of products. Recoveries ranged from 74% at 10 μg kg-1 for pork sausages to 96% at 40 μg kg-1 for meat pies.