Survey of Streptococcus equi subsp. equi in Imported Draft Horses and SeM Genetic Analysis of Isolates

Kei Nakajima, Kazufumi Kasuya, Fumi Oshima, Shohei Urushizaki, H. Ukai, R. Sumiyoshi, Katsunori Tagami, H. Niwa
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Abstract

imported Eleven asymptomatic horses from France tested positive on rPCR. Three S. equi strains were isolated from three individuals. We performed SeM genetic analysis on these three strains, in addition to four other strains isolated from Canadian draft horses and a Belgian riding horse that were developing strangles. The two strains from the French isolates were classified into a new SeM allelic group (SeM-200). Molecular phylogenetic tree analysis suggested that the French and Belgian strains were closely related and classified into different clus-ters from the Canadian strains. This study identified asymptomatic carriers of strangles among imported draft horses. ─
马链球菌亚种调查。进口驮马中的马及其分离株的SeM遗传分析
从法国进口的11匹无症状马的rPCR检测呈阳性。从3个个体中分离到3株马链球菌。我们对这三种菌株进行了SeM遗传分析,此外还有从加拿大役马和比利时骑马中分离的另外四种菌株,这些菌株正在发生勒死。从法国分离株中分离出的两个菌株被划分为一个新的SeM等位基因群(SeM-200)。分子系统进化树分析表明,法国和比利时菌株与加拿大菌株亲缘关系密切,属于不同的聚类。本研究在进口驮马中发现无症状的勒颈携带者。─
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