Sintesis Nanopartikel Perak Menggunakan Ekstrak Daun Salam (Syzygium polyanthum) Sebagai Bioreduktor Dan Uji Aktivitasnya Sebagai Antioksidan

P. Taba, Nadya Yuli Parmitha, Syahruddin Kasim
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引用次数: 8

Abstract

Silver nanoparticles have been synthesized by the reduction method using salam leaf extract (Syzygium polyanthum) as a reducing agent. The silver nanoparticles produced were then tested for their activity as antioxidants. The formation of silver nanoparticles was carried out by adding the leaf extract into the solution of AgNO3 and homogenized using a magnetic stirrer. A UV-Vis spectrophotometer was used to confirm the formation of silver nanoparticles. Particle Size Analyzer (PSA), Scanning Electron Microscope (SEM), X-Ray Diffractometer (XRD) and Fourir Transform Infra Red (FTIR) spectrometers were used to characterize the nanoparticles produced before being tested for antioxidant activity. The results showed that the absorbance value increased with increasing reaction contact time. The maximum uptake was obtained at wavelengths of 432-446 nm using a UV-Vis spectrophotometer. The particle size was determined using a PSA with an average particle size distribution of 45.7 nm. The average diameter of silver nanoparticles was 10.06 – 13.97 nm and the silver nanoparticles had rod-shapes. Functional groups that play a role in the synthesis of nanoparticles were –OH, –C=O, and –C-O groups. Silver nanoparticles inhibited free radicals as antioxidants with the IC50 value of 582.7 ppm.
以salam叶提取物(Syzygium polyanthum)为还原剂,采用还原法制备了纳米银。然后测试了生产出来的纳米银作为抗氧化剂的活性。将叶提取物加入AgNO3溶液中,用磁力搅拌器均质,制备纳米银颗粒。用紫外可见分光光度计对银纳米颗粒的形成进行了验证。采用粒径分析仪(PSA)、扫描电子显微镜(SEM)、x射线衍射仪(XRD)和傅里叶变换红外光谱仪(FTIR)对制备的纳米颗粒进行表征,然后进行抗氧化活性测试。结果表明,吸光度值随反应接触时间的增加而增加。紫外-可见分光光度计在432 ~ 446 nm波长处测得最大吸收率。采用PSA法测定其粒径,平均粒径分布为45.7 nm。银纳米粒子的平均直径为10.06 ~ 13.97 nm,呈棒状。在纳米颗粒合成中起作用的官能团是-OH、-C =O和-C -O基团。银纳米粒子对自由基具有抑制作用,IC50值为582.7 ppm。
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