The Influence Of Metal Ions On Cellulolytic Activities Of Fungal Isolates From Palm Biomass

Nigerian Journal of Biotechnology Pub Date : 2022-03-03 DOI:10.4314/njb.v38i2.7

D. C. Onyia, E. Onyeneke, T. Okunwaye, E. Okogbenin, N. U. Asiriuwa, J. U. Obibuzor, H. Anemene

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Abstract

Numerous metal ions and chemical compounds can influence the activity of some fungi. Some have inhibitory effects whereas others have enhancing effects. In this study, the effect of metallic chlorides; lead II chloride (PbCl2), potassium chloride (KCl), copper II chloride (CuCl2), calcium II chloride (CaCl2), manganese II chloride (MnCl2), iron III chloride (FeCl3) and mercury II chloride (HgCl2) were evaluated for their influence on the cellulolytic activity of seven (7) fungal isolates at a concentration of 0.005M at 30°C for four (4) days using 3, 5-dinitrosalicyclic acid (DNS) method. CaCl2 had the highest influence on the cellulolytic activity for all the fungal isolates as compared to the control. In the presence of CaCl2, Trichoderma species (C) showed a high cellulolytic activity of 2.28x10-5 mmol/min/ml, Rhizopus, Trichoderma and Aspergillus species (A, B and E) had activities of 1.72 x10-5 ,1.65 x10-5 and 1.68 x10-5 mmol/min/ml respectively. HgCl2 and CuCl2 had the least influenced on the cellulolytic activity of all the fungal isolates. This, therefore, suggests that calcium ion activates the cellulase activity of the fungal species while HgCl2, PbCl2, KCl, CuCl2, MnCl2 and FeCl3 had a retarding effect on the cellulolytic activity.

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金属离子对棕榈生物量真菌分离株纤维素分解活性的影响

许多金属离子和化合物可以影响一些真菌的活性。有些具有抑制作用，而另一些具有增强作用。在本研究中，金属氯化物的影响;采用3,5 -二硝基水杨环酸(DNS)法，研究了氯化铅(PbCl2)、氯化钾(KCl)、氯化铜(CuCl2)、氯化钙(CaCl2)、氯化锰(MnCl2)、氯化铁(FeCl3)和氯化汞(HgCl2)对7株真菌分离物在浓度为0.005M、30°C条件下4(4)天的纤维素水解活性的影响。与对照相比，CaCl2对所有真菌分离株的纤维素分解活性影响最大。在CaCl2的作用下，木霉(C)的纤维素水解活性为2.28 × 10-5 mmol/min/ml，根霉(a)、木霉(B)和曲霉(E)的纤维素水解活性分别为1.72 × 10-5、1.65 × 10-5和1.68 × 10-5 mmol/min/ml。HgCl2和CuCl2对各菌株的纤维素水解活性影响最小。因此，钙离子激活了真菌的纤维素酶活性，而HgCl2、PbCl2、KCl、CuCl2、MnCl2和FeCl3对纤维素酶的降解有阻滞作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nigerian Journal of Biotechnology

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