Antioxidant Activity Test of Ethanolic Extract of Tempuyung Leaves (Sonchus Arvensis L.) Based on Linoleic - Thiocyanic Method

Agus Suprijono, Dian Sulistiani, Sarosa Purwadi
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Abstract

Free radicals chemically have free electrons, free radicals tend to take electrons from other molecules, which then give rise to abnormal compounds. Antioxidants are substances that can delay or prevent free radical oxidation reactions in lipid oxidation. Tempuyung leaves contain flavonoids which can act as antioxidants. This research aims to determine the antioxidant activity of the ethanol extract of tempuyung leaves using the linoleic-thiocyanate method and to determine the total phenolic and total flavonoid content using the Folin-Chiocalteu reagent method. The results showed that the ethanol extract of tempuyung leaf extract had antioxidant activity. Percentage of antioxidant activity of 10 % extract = 82.91 %, 5 % extract = 70.07 %, 1 % extract = 57.70 %, Vitamin E 1 % = 70.2 1%. Total phenolic content of 10% extract = 0.48 %, 5 % extract = 0.25 % and 1 % extract = 0.04 %. The total flavonoid content of the extract is 10 % = 0.14 %, 5 % extract = 0.07 %, and 1 % extract = 0.02 %. Pearson correlation test showed that phenolic and flavonoid compounds greatly influenced the antioxidant activity of the ethanol extract of tempuyung leaves.
天普容叶乙醇提取物抗氧化活性试验基于亚油酸-硫氰酸法
自由基在化学上有自由电子,自由基倾向于从其他分子中获取电子,从而产生异常化合物。抗氧化剂是一种可以延缓或阻止脂质氧化过程中自由基氧化反应的物质。Tempuyung叶子含有类黄酮,可以作为抗氧化剂。本研究采用亚油酸-硫氰酸酯法测定天普容叶乙醇提取物的抗氧化活性,采用福林- chiocalteu试剂法测定其总酚和总黄酮含量。结果表明,天普容叶提取物乙醇提取物具有抗氧化活性。10%提取物的抗氧化活性百分比= 82.91%,5%提取物= 70.07%,1%提取物= 57.70%,维生素E 1% = 70.2 1%。10%提取物总酚含量= 0.48%,5%提取物= 0.25%,1%提取物= 0.04%。总黄酮含量为10% = 0.14%,5%提取物= 0.07%,1%提取物= 0.02%。Pearson相关检验表明,酚类和黄酮类化合物对天普容叶乙醇提取物的抗氧化活性有较大影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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