Selective, sensitive, and rapid liquid chromatography-tandem mass spectrometry method for determination of Guaifenesin in human plasma

Abstract

A simple, sensitive, selective, and rapid liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for quantitation of guaifenesin in human plasma using guaifenesin-d3 as internal standard (IS). Following solid phase extraction, the analyte was chromatographed using an isocratic mobile phase on a reversed-phase C18 column and analyzed by MS in multiple reaction-monitoring (MRM) mode (positive ion mode). The limit of quantitation for this method was 8 ng/mL and the linear dynamic range was 8–2200 ng/mL.