Isolation and Characterization of the Human Gene Homologous to the Drosophila Headcase (hdc) Gene in Chromosome Bands 6q23-q24, a Region of Common Deletion in Human Pancreatic Cancer

N. Makino, T. Yamato, H. Inoue, T. Furukawa, T. Abe, T. Yokoyama, T. Yatsuoka, S. Fukushige, Seiichi Orikasa, Tsuneo Takahashi, A. Horii
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引用次数: 20

Abstract

Deletions of the long arm of chromosome 6 (6q) are one of the most common chromosomal abnormalities in multiple human malignancies. Previously, we have identified three commonly deleted regions on 6q (6q21, 6q23-q24, and 6q26) in pancreatic cancer by loss of heterozygosity studies, suggesting the presence of one or more tumor suppressor genes on this chromosome arm. Using a combination of database search and cDNA library screening, we successfully isolated a transcript from 6q24. This mRNA encodes a protein consisting of 543 amino acids with homology to the Drosophila headcase (hdc) gene and, thus, is designated as hHDC. Northern analysis identified a ubiquitously expressed 5.6-kb transcript. Seventeen (81%) of 21 pancreatic cancer cell lines and four (80%) of five renal cell carcinoma cell lines showed low level expression, suggesting that the hHDC gene may play an important role in some human cancers.
人类胰腺癌常见缺失区6q23-q24染色体带果蝇头状(hdc)基因同源基因的分离与鉴定
6号染色体长臂缺失是多种人类恶性肿瘤中最常见的染色体异常之一。在此之前,我们通过杂合性缺失研究在胰腺癌中发现了6q上三个常见的缺失区域(6q21、6q23-q24和6q26),这表明在该染色体臂上存在一个或多个肿瘤抑制基因。通过数据库检索和cDNA文库筛选,我们成功地从6q24中分离出一个转录本。这种mRNA编码一种由543个氨基酸组成的蛋白质,与果蝇的头部(hdc)基因同源,因此被命名为hHDC。Northern分析鉴定出一个普遍表达的5.6 kb转录本。21株胰腺癌细胞系中有17株(81%)和5株肾细胞癌细胞系中有4株(80%)表达低水平,提示hHDC基因可能在某些人类癌症中发挥重要作用。
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