Studies on Midgut Microflora of Aedes Genus Mosquitoes from Badnapur Region (Jalna District), Areas of Maharashtra, India

Abstract

Mosquitoes are responsible insect as a vector to cause various diseases. If not cured on time it goes towards fatality. Majorly attacks of anopheles and aedes genus recently have an impact for causing Malaria, dengue, etc. We focused on aedes which was available in our local areas and easily identifiable with morphological characteristics of larvae and adults. First we collected larvae from storage and sewage water samples. Rearing was carried out in vitro with provision of different flower parts for feeding. All stages of life cycle were observed. For further study of gut Microflora adult stage mosquito dissected and exposed it’s gut to nutrient agar medium with saline. Characterization was done with different reagents, media and antibiotics. Amylase production detection assay, Catalase production detection assay, Citric acid utilization assay, Methyl red assay were positive. Phosphate solubilization activity detection assay, Arginine dihydrolase production detection assay, Indole Acetic acid (IAA) production detection assay, Voges Proskauer assay were negative. Isolate was resistant to all antibiotics such as Gentamycin, Kanamycin, Chloramphenicol, Vancomycin and Tetracycline. Gram staining was shown gram negative nature with pink colored colonies.