Development, Characterization, and In Vitro Evaluation of Chitosan and Alginate Microspheres Loaded Tetanus Toxoid Vaccine: A Comparative Study

International Journal of Green Nanotechnology Pub Date : 2011-07-27 DOI:10.1080/19430892.2011.571637

Saravanakumar Arthanari, Nachipalayam Muthusamy Ramaswamy, K. R. Mani

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引用次数: 1

Abstract

ABSTRACT The feasibility and utility of in vitro methods to assess the release pattern of chitosan and alginate microspheres containing experimental tetanus toxoid (TT) vaccines were evaluated. Herein, TT was stabilized and encapsulated in chitosan (TTCH) and alginate (TTAL) microspheres by a W/O/W multiple emulsion method using sucrose as a protein stabilizer. Optimization of processing parameters was studied with regard to polymer concentration, cross-linker concentration, and stirrer speed. The morphology of TTCH and TTAL microspheres prepared was smooth and spherical in shape with a diameter of around 10 μm. The vaccine entrapment and the in vitro release efficiency of both TTCH and TTAL were evaluated by differing stabilizer (sucrose) concentrations (5, 7, 10, and 12% w/v) for a period of 70 days. Antigen releases from microspheres were determined by enzyme-linked immunosorbent assay (ELISA). From the results it was concluded that 1 and 4% (polymers), 1700 and 8000 rpm (stirrer speed), and 5 and 8% (...

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壳聚糖和海藻酸盐微球负载破伤风类毒素疫苗的研制、表征和体外评价:比较研究

摘要:探讨壳聚糖和海藻酸盐微球体外评价破伤风类毒素(TT)疫苗释放模式的可行性和实用性。以蔗糖为蛋白质稳定剂，采用W/O/W复合乳液法将TT稳定并包封在壳聚糖(TTCH)和海藻酸盐(TTAL)微球中。对聚合物浓度、交联剂浓度、搅拌速度等工艺参数进行了优化研究。制备的TTCH和TTAL微球形貌光滑，呈球形，直径约为10 μm。通过不同的稳定剂(蔗糖)浓度(5、7、10和12% w/v)，对TTCH和TTAL的疫苗包埋和体外释放效率进行了为期70天的评估。采用酶联免疫吸附试验(ELISA)测定微球抗原释放量。结果表明:1和4%(聚合物)，1700和8000转/分(搅拌速度)，5和8%(搅拌速度)。

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International Journal of Green Nanotechnology

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