Combination effects of gallic acid and cyclosporine A during ischemia/ reperfusion on rat electrocardiogram parameters and arrhythmia

IF 0.3 Q4 PHYSIOLOGY

Physiology and Pharmacology Pub Date : 2021-07-10 DOI:10.32598/PPJ.25.2.80

N. Sadeghi, Samira Saadatfard, M. Dianat, H. Abedi, Khatereh Dehghani

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引用次数: 1

Abstract

Introduction: Myocardial ischemia leads to electrical disturbance in the heart because of reactive oxygen specious. This study aimed to investigate the effects of gallic acid and cyclosporine A (CsA) together on electrocardiogram parameters in myocardium following ischemia - reperfusion (I/R) in isolated hearts. Methods: In this research, 50 Wistar rats weighing 250-300g were randomly divided into the 5 following groups: control, sham and gallic acid (7.5, 15 and 30mg/kg) in combination with CsA (0.2μM). On the eleventh day, the hearts were removed and perfused with Krebs solution and ischemia was induced for 30min. Then, cyclosporine was administered for 10min at the 10 minutes before reperfusion and 10 minutes the beginning of reperfusion. By placing the electrode, the parameters of RR, PR, QT, TpeakTend, JT and QTcB interval, ST elevation, R, P, Q, S, T amplitude were recorded before ischemia and during reperfusion. Results: This study showed that RR, JT, interval, p duration, ST elevation and PVC numbers of control were increased during ischemia compared with sham and decreased using gallic acid (7.5, 15 and 30mg/kg) in combination with CsA. In addition, P, R, S, T amplitude during the ischemia were decreased in control compared with sham and increased with gallic acid (15mg/kg) in combination with CsA. Conclusion: In conclusion, the optimal combination of both drugs decreased arrhythmia occurrence while increased electrical velocity of conduction and wave amplitudes in isolated myocardium after ischemia reperfusion injury.

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缺血再灌注时没食子酸与环孢素A联合用药对大鼠心电图参数及心律失常的影响

导读:心肌缺血时由于活性氧的存在而引起心脏电干扰。本研究旨在探讨没食子酸和环孢素A (CsA)对离体心脏缺血再灌注(I/R)后心肌心电图参数的影响。方法:选取体重250 ~ 300g的Wistar大鼠50只，随机分为对照组、假药组和没食子酸(7.5、15、30mg/kg)联合CsA (0.2μM)组。第11天取出心脏，灌注Krebs溶液，诱导缺血30min。然后在再灌注前10分钟和再灌注开始10分钟给予环孢素10min。通过放置电极记录缺血前和再灌注时RR、PR、QT、TpeakTend、JT、QTcB间期、ST段抬高、R、P、Q、S、T幅值等参数。结果:与假手术相比，未食子酸(7.5、15和30mg/kg)联合CsA组缺血时，对照组的RR、JT、间期、p持续时间、ST段抬高和PVC数均升高，且降低。对照组缺血时P、R、S、T振幅较假手术组降低，未食子酸(15mg/kg)与CsA联合用药后P、R、S、T振幅升高。结论:综上所述，两种药物的最佳组合可降低离体心肌缺血再灌注损伤后心律失常的发生率，提高心肌的传导速度和波幅。

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来源期刊

Physiology and Pharmacology PHYSIOLOGY-

CiteScore

0.70

自引率

0.00%

发文量

期刊介绍： Physiology and Pharmacology is the official English publication of the Iranian Society of Physiology and Pharmacology. The journal publishes Review articles, Full-length original articles, Letter to editor and Short communications in physiology, pharmacology and related subjects. The aim of this journal is to provide a medium of scientific communication for investigators in the field of Physiology and Pharmacology. The editors will welcome original basic and applied research articles from Physiologists and Pharmacologists. Articles should be in English language. The papers submitted to this journal must not be Published or under consideration for publication elsewhere. Physiology and Pharmacology is an open access journal which means that all contents is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search or link to the full text of the articles in this journal without asking prior permission from the publisher or the author.