Pharmacognostic study of cetraria islandica (L.) Ach. thalli made in Ukraine

Q3 Pharmacology, Toxicology and Pharmaceutics

ScienceRise: Pharmaceutical Science Pub Date : 2022-10-31 DOI:10.15587/2519-4852.2022.265019

A. Shpychak, O. Khvorost

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Abstract

The aim was to conduct a pharmacognostic study of C. islandica thalli harvested in Ukraine. Material and methods. The study included seven series of C. islandica thalli harvested in Ukraine. Pharmacognostic research was conducted by using generally accepted methods. Quantitative determination of total polyphenols was performed by a spectrophotometric method in terms of pyrogallol and dry raw materials following the requirements of the SPhU 2.0 monograph. The component composition of flavonoid compounds and carboxylic acids was performed by HPLC. Results. The pharmacognostic study of 7 series of the C. islandica raw materials was performed. Morphometric parameters were determined, including the fractional composition according to the size of thalli in each series. For the first time, fractions of the mineral and foreign organic matter were separated. The content of mineral foreign matter ranged from 0.22 ± 0.01 % to 2.80 ± 0.12 %; the content of organic foreign matter ranged from 0.15 ± 0.01 % to 2.14 ± 0.11 %. Due to the total foreign matter content, 6 series of the raw materials fulfilled the requirements of the SPhU 2.0 monograph. The accordance of morphological and anatomical description of the series to the requirements of the SPhU 2.0 monograph is shown. New distinctive diagnostic features of the morphological structure were found, namely: coalescence of blades with forming a membrane and branching of cilia along the edge of blades. For the first time for the series of raw materials harvested in Ukraine, the quantitative content of the sum of polyphenols in terms of pyrogallol and dry raw materials was determined, which ranged from 1.21 ± 0.05 % to 1.73 ± 0.04 %. For the first time for C. islandica thalli, the presence of flavonoid compounds: quercetin, luteolin, kaempferol and rutin, and carboxylic acids: sinapic, trans-cinnamic and quinic acids was determined. Conclusions. The obtained results can be used as a basis for the relevant sections of the national part of the monograph "Cetraria Iceland" in SPhU 2.0

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天竺葵的生药学研究课时。萨利是乌克兰制造的

目的是对在乌克兰收获的岛孢菌体进行生药学研究。材料和方法。该研究包括在乌克兰收获的七个系列岛芽孢杆菌。采用普遍接受的方法进行生药学研究。根据SPhU 2.0专著的要求，用分光光度法对邻苯三酚和干燥原料进行了总多酚的定量测定。用高效液相色谱法测定了黄酮类化合物和羧酸的组成。结果。对7个系列岛楝原料进行了生药学研究。确定形态计量学参数，包括每个系列中根据菌体大小的分数组成。矿物和外来有机物的组分第一次被分离。矿物异物含量范围为0.22±0.01% ~ 2.80±0.12%;有机异物含量范围为0.15±0.01% ~ 2.14±0.11%。由于总杂质含量，6个系列的原料满足SPhU 2.0各论的要求。该系列的形态和解剖描述符合SPhU 2.0专著的要求。发现了新的形态结构特征，即叶片合并形成膜和沿叶片边缘的纤毛分支。首次对乌克兰收获的系列原料进行了以邻苯三酚和干原料为单位的多酚和的定量含量测定，其含量范围为1.21±0.05% ~ 1.73±0.04%。首次测定了岛菜菌体中黄酮类化合物槲皮素、木犀草素、山奈酚和芦丁的含量，羧酸类化合物辛酸、反式肉桂酸和奎宁酸的含量。结论。所得结果可作为SPhU 2.0专著《冰岛塞特里亚》(Cetraria Iceland)国家部分相关章节的依据

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