The effect of parathyroid hormone on osteogenic activities of human osteosarcoma cell line SaOS-2

Q3 Medicine

中华地方病学杂志 Pub Date : 2011-05-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2011.03.014

Liu Kang-kang, Huo Liang-liang, Zhao Li-jun, Shi Yu-xia, Zhang Li-wei, Pei Junrui, Geng Li-bin, Gao Yanhui

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Abstract

Objective To observe the effects of recombinant human parathyroid hormone 1 to 34(referred to as hPTH) on the expression level of alkaline phosphatase(ALP) and bone gla protein(BCP) in human osteosarcoma cell line SaOS-2(referred to as SaOS-2 cells). Methods SaOS-2 cells were subcultured and treated with 1, 10 and 100 nmol/L hPTH for 12, 24 and 48 h. Total cellular RNA was extracted, cDNA was synthesized by reverse doses of hPTH, different duration of action, and their interaction on the expression level of ALP mRNA of SaOS-2 cells was significantly different(F = 29.32, 2.92, 7.64, all P < 0.05). The expression level of ALP mRNA(0.78 ± 0.43, 0.71 ± 0.05, 0.75 ± 0.19, 0.76 ± 0.14) of SaOS-2 cells after treatment with 0, 1, 10 and 100 nmol/L hPTH for 48 h was lower than those of treated for 12 h(1.01 ± 0.16, 1.37 ± 0.38, 1.49 ± 0.16, 2.52 ± 0.70, all P< 0.05) and 24 h (1.80 ± 0.47, 1.30 ± 0.36, 1.27 ± 0.17, 1.17 ± 0.11, all P< 0.05). The expression level of ALP mRNA of SaOS-2 cells after treatment with 100 nmol/L hPTH for 12 hours was higher than that of the control(P < 0.05); the expression level of ALP mRNA of SaOS-2 cells after treatment with 1, 10 and 100 nmol/L hPTH for 24 h interaction on the expression level of BGP mRNA of SaOS-2 were significantly different (F = 8.26, 10.33, 5.51, all P< 0.05). The expression level of BGP mRNA(1.17 ± 0.28, 0.98 ± 0.08, 0.92 ± 0.17 and 0.84 ± 0.59) of SaOS2 cells after treatment with 0, 1, 10 and 100 nmol/L hPTH for 48 h was lower than those of treated for 12 h( 1.01 ± 0.14, 1.21 ± 0.18, 1.34 ± 0.30, 1.68 ± 0.62, all P< 0.05), and 24 h(1.71 ± 0.35, 1.41 ± 0.47, 1.28 ± 0.31 and 1.01 ± 0.18, all P < 0.05). The expression level of BGP mRNA of SaOS-2 cells after treatment with 100 nmol/L hPTH for 12 h was higher than that of those groups treated with 0 and 1 nmol/L hPTH(all P< 0.05). The expression level of BGP mRNA of SaOS-2 cells after treatment with 10 and 100 nmol/L hPTH for 24 h and 48 h was lower than those of the control(all P < 0.05). The expression level of BGP mRNA of SaOS-2 cells after treatment with 100 nmol/L hPTH for 24 hours was lower than that the group treated with 1 nmol/L hPTH(P < 0.05). Conclusions In vitro, hPTH significantly enhances osteogenic activities of human osteoblast in a short time, however, with prolonged stimulation time, osteogenic activity can show a downward trend. Key words: Teriparatide; Osteoblasts; Alkaline phosphatase; Osteocalcin

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甲状旁腺激素对人骨肉瘤细胞系SaOS-2成骨活性的影响

目的观察重组人甲状旁腺激素1 ~ 34(以下简称hPTH)对人骨肉瘤细胞系SaOS-2(以下简称SaOS-2细胞)碱性磷酸酶(ALP)和骨玻璃蛋白(BCP)表达水平的影响。方法对SaOS-2细胞进行传代培养，分别用1、10和100 nmol/L hPTH处理12、24和48 h，提取细胞总RNA，合成cDNA，不同剂量hPTH作用时间对SaOS-2细胞ALP mRNA表达水平的交互作用有显著差异(F = 29.32、2.92、7.64，均P < 0.05)。0、1、10、100 nmol/L hPTH作用48 h后SaOS-2细胞ALP mRNA表达量(0.78±0.43、0.71±0.05、0.75±0.19、0.76±0.14)低于作用12 h(1.01±0.16、1.37±0.38、1.49±0.16、2.52±0.70，均P< 0.05)和作用24 h(1.80±0.47、1.30±0.36、1.27±0.17、1.17±0.11，均P< 0.05)。100 nmol/L hPTH作用12 h后SaOS-2细胞ALP mRNA表达量显著高于对照组(P < 0.05);1、10和100 nmol/L hPTH作用24 h后SaOS-2细胞ALP mRNA表达量对SaOS-2细胞BGP mRNA表达量的影响均有显著差异(F = 8.26、10.33、5.51，均P< 0.05)。0、1、10、100 nmol/L hPTH作用48 h后SaOS2细胞BGP mRNA表达量(1.17±0.28、0.98±0.08、0.92±0.17、0.84±0.59)低于作用12 h(1.01±0.14、1.21±0.18、1.34±0.30、1.68±0.62，均P< 0.05)和作用24 h(1.71±0.35、1.41±0.47、1.28±0.31、1.01±0.18，均P< 0.05)。100 nmol/L hPTH作用12 h后，SaOS-2细胞BGP mRNA表达量显著高于0和1 nmol/L hPTH组(均P< 0.05)。10和100 nmol/L hPTH作用24 h和48 h后SaOS-2细胞BGP mRNA表达量均低于对照组(均P < 0.05)。100 nmol/L hPTH作用24 h后SaOS-2细胞BGP mRNA表达量低于1 nmol/L hPTH组(P < 0.05)。结论在体外，hPTH能在短时间内显著增强人成骨细胞的成骨活性，但随着刺激时间的延长，成骨活性呈下降趋势。关键词:特立帕肽;成骨细胞;碱性磷酸酶;骨钙素

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.