Calibration and Evaluation of Quantitative Antibody Titers for Measles Virus by Using the BioPlex 2200.

Q2 Biochemistry, Genetics and Molecular Biology
Clinical and Vaccine Immunology Pub Date : 2017-01-05 Print Date: 2017-01-01 DOI:10.1128/CVI.00269-16
Todd F Hatchette, Heidi Scholz, Shelly Bolotin, Natasha S Crowcroft, Colleen Jackson, Elizabeth McLachlan, Alberto Severini
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引用次数: 12

Abstract

The BioPlex 2200 (Bio-Rad Laboratories, Hercules, CA) is a rapid, automated platform, which can screen large numbers of specimens for antibodies to measles, mumps, rubella, and varicella. Although approved for producing qualitative results, in this study we validated the test (off-label) to allow reporting of quantitative results. To do this, we used the third anti-measles World Health Organization standard to generate a calibration curve that allowed relative fluorescence intensity to be translated into quantitative antibody titer (antibody units [AU]/ml). The results from the BioPlex 2200 and the reference plaque reduction neutralization test (PRNT) exhibited a reasonable correlation following an exponential function, but correlation was poor in low-titer samples. Using a receiver operating characteristics analysis, an equivocal zone for the BioPlex 2200 was established between ≥0.13 and <1.10 AU/ml to achieve 100% specificity (95% confidence interval [CI] = 83.2 to 100%) and 100% sensitivity (95% CI = 93.5 to 100%) versus PRNT. By determining an equivocal range requiring confirmation by PRNT, we can avoid underestimating the levels of immunity through false-negative results and optimize methods for seroepidemiological studies.

应用BioPlex 2200对麻疹病毒定量抗体效价进行校正和评价。
BioPlex 2200 (Bio-Rad Laboratories, Hercules, CA)是一种快速、自动化的平台,可筛选大量麻疹、腮腺炎、风疹和水痘抗体标本。虽然已批准产生定性结果,但在本研究中,我们验证了该测试(非标签)以允许报告定量结果。为此,我们使用世界卫生组织第三种抗麻疹标准来生成校准曲线,该曲线允许将相对荧光强度转换为定量抗体滴度(抗体单位[AU]/ml)。BioPlex 2200和参考斑块减少中和试验(PRNT)的结果在指数函数下显示出合理的相关性,但在低滴度样品中相关性较差。使用接收器工作特性分析,BioPlex 2200在≥0.13和之间建立了一个模糊区域
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来源期刊
Clinical and Vaccine Immunology
Clinical and Vaccine Immunology 医学-传染病学
CiteScore
2.88
自引率
0.00%
发文量
0
审稿时长
1.5 months
期刊介绍: Cessation. First launched as Clinical and Diagnostic Laboratory Immunology (CDLI) in 1994, CVI published articles that enhanced the understanding of the immune response in health and disease and after vaccination by showcasing discoveries in clinical, laboratory, and vaccine immunology. CVI was committed to advancing all aspects of vaccine research and immunization, including discovery of new vaccine antigens and vaccine design, development and evaluation of vaccines in animal models and in humans, characterization of immune responses and mechanisms of vaccine action, controlled challenge studies to assess vaccine efficacy, study of vaccine vectors, adjuvants, and immunomodulators, immune correlates of protection, and clinical trials.
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