Structural and functional organisation of the phytopathogenic fungi Phoma sp.1 mitochondrial genome

Abstract

The article presents the results of the mitochondrial DNA (30 837 n. r.) sequencing of the phytopathogenic fungi Phoma sp.1 – causative agent of Phoma blight of the pine and spruce plants cultivated in the forest nurseries. Annotation of the Phoma sp.1 mitochondrion showed 43 coding loci. Potential open reading frames (orf89, orf87, orf76 and orf108) and gene introns (cox3, nad1) are described. A comparative single genes analysis in the NCBI GenBank database showed, that Phoma sp.1 belongs to the Didymella spp., which can have Phoma anamorph. It has been shown that mitohondrial genes can be used as DNA markers for the diagnosis of Phoma and phoma-like fungi. Analysis of the mitochondrial synthenia of Phoma sp.1 and a related species (including phoma-like fungi), revealed significant structural rearrangements in mtDNA during phylogenesis.