Evaluation of antimicrobial activity and in vitro safety of the methanolic extract of Streptomyces manipurensis soil isolate H21 for potential industrial applications

Yomna N. Elkholy, W. Elkhatib, K. Aboshanab, M. Aboulwafa, N. Hassouna
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引用次数: 3

Abstract

Streptomyces manipurensis isolate H21 was recovered from a soil sample in Cairo, Egypt. Cell-free culture supernatant of Streptomyces manipurensis isolate H21 previously showed antifungal and broad-spectrum antibacterial activity against some Gram-positive, Gram-negative, MDR, and ESBL producer pathogens as well as some reference strains. The present study aimed at investigating antimicrobial and cytotoxic activities of its crude methanolic extract. The antibacterial activity of the crude methanolic extract was determined using broth-dilution method against Staphylococcus aureus ATCC 43300, Klebsiella oxytoca ATCC 700324, Klebsiella pneumoniae ATCC 700603, Klebsiella pneumoniae ATCC BAA-1705 and 2 MDR uropathogens. The minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of the crude extract against the tested bacteria were in the range of 5-10 mg/mL and 10 mg/mL, respectively. The results revealed that the CD50 value of the extract was 1.17 mg/mL, against Caco-2 cell line, indicating in vitro safety and low cytotoxicity. Accordingly, Streptomyces manipurensis isolate H21 would be an excellent source of relatively safe and potent antibacterial agent.
马粪链霉菌土壤分离物H21甲醇提取物的抑菌活性及体外安全性评价
从埃及开罗的土壤样品中分离得到了手性链霉菌H21。此前,曼嘌呤链霉菌分离株H21的无细胞培养上清液对一些革兰氏阳性、革兰氏阴性、耐多药和ESBL产生菌以及一些参比菌株显示出抗真菌和广谱抗菌活性。本研究旨在研究其粗甲醇提取物的抑菌活性和细胞毒活性。采用肉汤稀释法测定粗甲醇提取物对金黄色葡萄球菌ATCC 43300、氧化克雷伯菌ATCC 700324、肺炎克雷伯菌ATCC 700603、肺炎克雷伯菌ATCC BAA-1705和2种耐多药尿路病原菌的抑菌活性。粗提物对被试细菌的最低抑菌浓度(mic)为5 ~ 10 mg/mL,最低杀菌浓度(MBCs)为10 mg/mL。结果表明,该提取物对Caco-2细胞株的CD50值为1.17 mg/mL,表明其体外安全性和低细胞毒性。因此,手性链霉菌H21是一种相对安全有效的抗菌药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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