Depth-Sensitive Raman Spectroscopy of Intact Formalin-Fixed and Paraffin-Embedded Tissue Blocks for Objective Diagnosis of Cancer- An Exploratory Study

K. M. Khan, Hemant Krishna, C. V. Kulkarni, S. Majumder
{"title":"Depth-Sensitive Raman Spectroscopy of Intact Formalin-Fixed and Paraffin-Embedded Tissue Blocks for Objective Diagnosis of Cancer- An Exploratory Study","authors":"K. M. Khan, Hemant Krishna, C. V. Kulkarni, S. Majumder","doi":"10.6000/1927-7229.2016.05.04.4","DOIUrl":null,"url":null,"abstract":"Histopathology, the current “gold standard”, is prone to human errors as it depends on expert interpretation of the microscopically derived cellular and sub-cellular information for tissue diagnosis. Further, this light microscope based approach requires preparation of appropriately stained specimens of micro-thin tissue sections from the formalin-fixed and paraffin-embedded (FFPE) blocks of tissue samples. We report a method that provides quantitative feedback about tissue diagnosis by measuring depth-sensitive Raman spectra from the intact FFPE tissue blocks without requiring preparation of any thin tissue sections or any other processing. The FFPE blocks of pathologically certified cancerous and normal breast tissues were used for validating the approach. The measured depth-sensitive Raman spectra were mathematically de-paraffinized for retrieving the characteristic tissue Raman signatures using scaled-subtraction. A multivariate analysis of the scaled-subtracted, depth-sensitive Raman spectra employing a probability-based diagnostic algorithm developed using the framework of sparse multinomial logistic regression (SMLR) provided a sensitivity and specificity of up to 100% towards cancer based on leave-one-block-out cross validation. The results of this exploratory study suggest that depth-sensitive Raman spectroscopy along with a multivariate statistical algorithm can provide a valuable alternate diagnostic modality in clinical pathology setting for discriminating cancerous from normal FFPE tissue blocks.","PeriodicalId":14957,"journal":{"name":"Journal of Analytical Oncology","volume":"100 1","pages":"153-163"},"PeriodicalIF":0.0000,"publicationDate":"2016-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Analytical Oncology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.6000/1927-7229.2016.05.04.4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

Abstract

Histopathology, the current “gold standard”, is prone to human errors as it depends on expert interpretation of the microscopically derived cellular and sub-cellular information for tissue diagnosis. Further, this light microscope based approach requires preparation of appropriately stained specimens of micro-thin tissue sections from the formalin-fixed and paraffin-embedded (FFPE) blocks of tissue samples. We report a method that provides quantitative feedback about tissue diagnosis by measuring depth-sensitive Raman spectra from the intact FFPE tissue blocks without requiring preparation of any thin tissue sections or any other processing. The FFPE blocks of pathologically certified cancerous and normal breast tissues were used for validating the approach. The measured depth-sensitive Raman spectra were mathematically de-paraffinized for retrieving the characteristic tissue Raman signatures using scaled-subtraction. A multivariate analysis of the scaled-subtracted, depth-sensitive Raman spectra employing a probability-based diagnostic algorithm developed using the framework of sparse multinomial logistic regression (SMLR) provided a sensitivity and specificity of up to 100% towards cancer based on leave-one-block-out cross validation. The results of this exploratory study suggest that depth-sensitive Raman spectroscopy along with a multivariate statistical algorithm can provide a valuable alternate diagnostic modality in clinical pathology setting for discriminating cancerous from normal FFPE tissue blocks.
完整的福尔马林固定和石蜡包埋组织块的深度敏感拉曼光谱客观诊断癌症-一项探索性研究
组织病理学是目前的“金标准”,由于它依赖于专家对显微镜下细胞和亚细胞信息的解释来进行组织诊断,因此容易出现人为错误。此外,这种基于光学显微镜的方法需要从组织样本的福尔马林固定和石蜡包埋(FFPE)块中制备适当染色的微薄组织切片标本。我们报告了一种方法,该方法通过测量完整FFPE组织块的深度敏感拉曼光谱来提供组织诊断的定量反馈,而无需制备任何薄组织切片或任何其他处理。病理证实的癌性和正常乳腺组织的FFPE块用于验证该方法。对测量的深度敏感拉曼光谱进行数学脱石蜡处理,利用比例减法提取组织特征拉曼特征。采用稀疏多项式逻辑回归(SMLR)框架开发的基于概率的诊断算法,对标度减去的深度敏感拉曼光谱进行了多变量分析,基于留一块交叉验证,对癌症的敏感性和特异性高达100%。这项探索性研究的结果表明,深度敏感拉曼光谱以及多元统计算法可以在临床病理环境中为区分癌变和正常FFPE组织块提供有价值的替代诊断模式。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信