PhD, FIBiol John Hay , MD, FRCPath, FRCOphth David V. Seal , BSc Ruth Connor , PhD Peter A. Simmons , PhD Alan Tomlinson
{"title":"Disinfection and sonication: Effect on association of Acanthamoeba cysts with an ionic, high-water content hydrogel contact lens","authors":"PhD, FIBiol John Hay , MD, FRCPath, FRCOphth David V. Seal , BSc Ruth Connor , PhD Peter A. Simmons , PhD Alan Tomlinson","doi":"10.1016/S0141-7037(95)80042-5","DOIUrl":null,"url":null,"abstract":"<div><p>Sets of 16 quarter segments of ionic, high-water content hydrogel contact lenses were incubated with mature cysts of <em>Acanthamoeba castellanii</em>. Lens pieces with associated amoebae were then incubated overnight at 25°C with one of the following: Prymesoak, Sofab, 10.10, ReNu, Optifree or sterile isotonic saline. Other lens pieces were sonicated at a frequency of 110 cycles/sec for 5 min in one of these systems or sonicated and then incubated overnight in the disinfectant. Subsequent culture for amoebal viability indicated that only Prymesoak and 10:10 provided 100% efficacy of action against lens-associated <em>Acanthamoeba</em> cysts, but Prymesoak required overnight incubation for such an effect. Sonication before incubation did not significantly improve the acanthamoebicidal properties of the disinfecting systems.</p></div>","PeriodicalId":100842,"journal":{"name":"Journal of The British Contact Lens Association","volume":"18 1","pages":"Pages 5-8"},"PeriodicalIF":0.0000,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0141-7037(95)80042-5","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of The British Contact Lens Association","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0141703795800425","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
Abstract
Sets of 16 quarter segments of ionic, high-water content hydrogel contact lenses were incubated with mature cysts of Acanthamoeba castellanii. Lens pieces with associated amoebae were then incubated overnight at 25°C with one of the following: Prymesoak, Sofab, 10.10, ReNu, Optifree or sterile isotonic saline. Other lens pieces were sonicated at a frequency of 110 cycles/sec for 5 min in one of these systems or sonicated and then incubated overnight in the disinfectant. Subsequent culture for amoebal viability indicated that only Prymesoak and 10:10 provided 100% efficacy of action against lens-associated Acanthamoeba cysts, but Prymesoak required overnight incubation for such an effect. Sonication before incubation did not significantly improve the acanthamoebicidal properties of the disinfecting systems.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
