Diverse Pattern of Cytokine Production, their Functional Association andmRNA Expression in Tuberculosis Patients with Diabetes Mellitus and theirHousehold Contacts

P. Meenakshi, Sivangala Ramya, A. Madhavilatha, J. Lavanya, G. Sumanlatha
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引用次数: 1

Abstract

Cytokine genes are associated with their altered production and play a significant role in tuberculosis patients with diabetes mellitus and their household contacts. We aimed to study the influence of IL-1β and IL-6 gene polymorphisms on cytokine gene production and to evaluate their mRNA expression in M.tbAg85A stimulated culture supernatants of tuberculosis patients with diabetes mellitus, pulmonary tuberculosis patients, household contacts and diabetes individuals. Enzyme linked immunosorbent assay was used to estimate cytokine production in the culture supernatants and TRIZOL method for mRNA expression. Cytokine production was low in all the subjects when compared to the healthy controls and IL-6 levels increased with treatment. The CC genotype of IL-6 -174 G/C was associated with its low production in household contacts of tuberculosis patients with diabetes mellitus and pulmonary tuberculosis patients. The mRNA expression of IL-1β increased and that of IL-6 decreased in tuberculosis patients with diabetes mellitus, while that of IL-6 decreased with treatment in pulmonary tuberculosis patients; however, there was no significant difference in the household contacts. The cytokine levels were almost similar in patients and their household contacts at the time of diagnosis suggesting that even the household contacts might be infected thus aiding in protecting the high-risk individuals by their prior identification.
结核糖尿病患者及其家庭接触者细胞因子产生模式、功能关联及mrna表达
细胞因子基因与其改变的产生相关,并在合并糖尿病的肺结核患者及其家庭接触者中发挥重要作用。本研究旨在研究结核合并糖尿病患者、肺结核患者、家庭接触者和糖尿病患者M.tbAg85A刺激培养上清中IL-1β和IL-6基因多态性对细胞因子基因产生的影响,并评价其mRNA表达。酶联免疫吸附法测定细胞因子在培养上清液中的产量,TRIZOL法测定mRNA的表达。与健康对照组相比,所有受试者的细胞因子产生都较低,IL-6水平随治疗而升高。白细胞介素-6 -174 G/C基因型与肺结核患者合并糖尿病和肺结核患者家庭接触者白细胞介素-6 -174 G/C的低产量有关。结核合并糖尿病患者IL-1β mRNA表达升高,IL-6 mRNA表达降低,而结核合并糖尿病患者IL-6 mRNA表达随治疗而降低;然而,在家庭接触方面没有显著差异。在诊断时,患者及其家庭接触者的细胞因子水平几乎相似,这表明即使是家庭接触者也可能被感染,从而有助于通过事先识别来保护高危个体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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