Design an Enzyme-Less Sensitive Sensor for Cholesterol Detection Based on Poly Brucine/Polyvinylpyrrolidone Film

Sensor Letters Pub Date : 2020-11-01 DOI:10.1166/sl.2020.4294

Nahid Taghiee Karaji, Samineh Kaki, A. Babakhanian

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Abstract

In the current study, the main focus is designing a new enzyme-less modified electrode based on the electro deposition of Poly Brucine/Polyvinylpyrrolidone on pencil graphite electrode and its utilizing for cholesterol quantification. The electrochemical responses of the proposed sensor were recorded using cyclic and square wave voltammetry techniques for detection of cholesterol at optimized pH =2. The morphology investigations of the modified surfaces were performed by scanning electron microscopy and the obtained images verified Nano formations in the structure of the sensor. The proposed electrochemical sensor revealed a good electro catalytic response to cholesterol concerning the parameters α = 0.83, log Ks = 3.11 and Γ = 8.55 × 10T8. Besides, the calibration plot was acquired with a linear portion in the concentration ranges of 25 to 95 nmol L−1 (nM) and the detection limit (LOD = 8.28 nM). The designed sensor demonstrated the reproducible and repeat-able outputs, the high sensitivity and stability in analyzing cholesterol as well as recoveries in blood serum samples without any side serious interferences.

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基于聚马钱子碱/聚乙烯吡咯烷酮薄膜的低酶敏感胆固醇传感器设计

在目前的研究中，主要是设计一种新的基于聚马子碱/聚乙烯吡罗烷酮在铅笔石墨电极上电沉积的无酶修饰电极，并将其用于胆固醇的定量。利用循环伏安法和方波伏安法记录了该传感器在优化pH =2时检测胆固醇的电化学响应。通过扫描电子显微镜对修饰表面进行形貌研究，获得的图像验证了传感器结构中的纳米结构。在α = 0.83, log Ks = 3.11， Γ = 8.55 × 10T8的条件下，电化学传感器对胆固醇具有良好的电催化反应。校正图在25 ~ 95 nmol L−1 (nM)范围内呈线性关系，检出限为8.28 nM。所设计的传感器具有重现性和可重复性，在分析胆固醇和血清样品的回收率方面具有高灵敏度和稳定性，无任何副作用严重干扰。

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来源期刊

Sensor Letters 工程技术-电化学

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.