Assessment of the photosensitizer efficacy after determination of threshold doses initiating erythrocyte hemolysis

中国激光医学杂志 Pub Date : 2021-11-27 DOI:10.37895/2071-8004-2021-25-2-41-47

Y. Alekseev, O. V. Mislavsky, V. Duvanskiy, R. A. Duvanskiy, N. V. Drozdova

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引用次数: 2

Abstract

Purpose: To find out a technique for determining the effectiveness of porphyrin-type photosensitizers with concentrations which cause hemolysis in erythrocytes (hemolysis threshold). The hemolysis threshold is found under the following conditions: irradiation in the Soret band with standard parameters – power density, exposure dose, irradiation time.Material and methods. Phototherapeutic device “AST” (LLC “Pankov-medical”) – average power 0.5 W, wavelength ≈ 405 nm. Rat blood erythrocytes. “Multiscan MS” device manufactured by Labsystems, Finland. Preparation “Dimegin”, hematoporphyrin derivative; preparation “Photoditazine” (LLC “VETA-GRAND”), chlorine E6 derivative.Results. On analyzing the results obtained after experimentation with the abovementioned two preparations, it was shown that the developed technique has a high efficacy. It was also shown that the studied photosensitizers are equally effective when irradiated in the Soret band.Conclusion. The developed technique is simple and easy to use. It helps to assess the efficacy of various photosensitizers thus, facilitating the selection of their necessary concentrations for managing different diseases.

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在确定启动红细胞溶血的阈剂量后评估光敏剂的功效

目的:建立一种测定红细胞溶血浓度(溶血阈值)的卟啉型光敏剂有效性的方法。溶血阈值是在以下条件下发现的:在Soret波段照射，标准参数-功率密度，照射剂量，照射时间。材料和方法。光疗装置“AST”(LLC“Pankov-medical”)-平均功率0.5 W，波长≈405 nm。大鼠红细胞。芬兰Labsystems公司生产的“Multiscan MS”设备。血卟啉衍生物“Dimegin”的制备;制备“photodiazine”(LLC“VETA-GRAND”)，氯E6衍生物。对上述两种制剂的实验结果进行了分析，表明所开发的技术具有较高的效率。研究还表明，所研究的光敏剂在Soret波段照射时具有相同的效果。所开发的技术简单易行。它有助于评估各种光敏剂的功效，从而促进选择治疗不同疾病所需的浓度。

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来源期刊

中国激光医学杂志

自引率

0.00%

发文量

5650

期刊介绍： The main columns of "Chinese Journal of Laser Medicine & Surgery" include treatises, which report the latest research results in basic research and clinical trials in the field of laser medicine; comprehensive reviews of the latest research progress in laser medicine at home and abroad. In addition, there are short reports and excerpts from foreign journals, conference news and other columns. The journal has published a large number of papers on basic research on laser medicine and applied research in various clinical subjects, aiming to play a positive role in promoting the application of laser in medicine and improving the level of laser medicine research.