Sub-Acute Toxicity Studies of Alchornea cordifolia Leaf Extract in SwissAlbino Rats

Abstract

The ethanolic leaf extract of Alchornea cordifolia (Schum. and Thonn.) Mull. Arg (Euphorbiaceae), a widely used traditional medicinal plant was assessed for possible sub-acute toxicity in Swiss albino rats. The rats were randomly distributed into five groups of four animals each. The groups were respectively administered 125, 250, 500 and 750 mg/kg body weight ethanolic leaf extract of Alchornea cordifolia intra peritoneally daily for (two weeks) 14 days. Normal saline was administered to the control group according to their body weights. The experimental animals were observed for another 14 days before the termination of the experiment. The weight of the animals was recorded daily throughout the duration of the study. The number of deaths in any group was recorded. All the surviving animals were sacrificed after 28 days. Blood samples were collected for biochemical and haematological analysis. Selected organs of the animals i.e., liver and kidney of both the dead and sacrificed animals were removed and stored in 10% formal saline ready for histopathological analysis. Administration of Alchornea cordifolia (0.125- 0.75 g/kg, po daily) for two weeks (14 days) did not affect significantly the relative organ weights, blood chemistry and renal function. Histology of liver and kidney at dose levels up to 0.5 g/kg was normal and similarto vehicletreated controls. However, liver sections of mice treated with 0.75 g/kg Alchornea cordifolia ethanolic leaf extract showed cloudy swelling of hepatocytes with vascular degeneration. These results suggest that Alchornea cordifolia is relatively non-toxic but has the propensity to induce hepatic injury at high doses.