Genotoxicity Of Petroleum Refinery Waste Water In Nigeria

Global Journal of Environmental Sciences Pub Date : 2005-02-08 DOI:10.4314/GJES.V3I1.2429

G. Obute, L. Osuji, Cordelia Kalio

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引用次数: 1

Abstract

The genotoxicity of petroleum refinery wastewater was investigated with the Allium test. 10 medium – sized Allium cepa L. bulbs of the pink variety were induced to sprout roots in distilled water and variously assaulted for 48 hours with different concentrations (v/v) of wastewater from the refinery. Serial dilutions of 20%, 40%. 60%, 80% and 100% were used while distilled water served as control. After the assault, roots were harvested from each treatment sample and assayed for genotoxic effects with the acetocarmine squash technique. Results showed that the wastewater induced statistically highly significant (P ≤ 0.001) mitodepressive effects which were dose-dependent, culminating in total mitotic inhibition at 100% v/v concentration. Other aberrations including stickiness of chromosomes, erosion of chromatin, vagrant chromosomes, fragments and anaphase bridges were induced at higher concentrations (60%-80%) of wastewater. Lower concentrations (20%-40%) induced c-mitosis as the major aberration. The advantages of genotoxicity screening over general toxicity testing in environmental monitoring was highlighted. Key Words: Allium test, pollution, genotoxicity, chromosome, wastewater. Global Journal of Environmental Sciences Vol.3(1&2) 2004: 55-58

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尼日利亚炼油厂废水的遗传毒性

采用葱试验研究了石油炼制废水的遗传毒性。采用不同浓度(v/v)的精炼厂废水，在蒸馏水中培养10株中等大小的粉红品种葱属鳞茎，诱导其生根48小时。连续稀释20%，40%。分别为60%、80%和100%，蒸馏水为对照。攻击后，从每个处理样品中收获根，并使用乙酰胭脂红南瓜技术检测基因毒性效应。结果表明，废水诱导的有丝分裂抑制效应具有统计学高度显著(P≤0.001)，且呈剂量依赖性，最终达到100% v/v浓度的有丝分裂抑制。其他畸变包括染色体黏性、染色质侵蚀、染色体漂移、片段和后期桥在较高浓度(60%-80%)的废水中引起。较低浓度(20%-40%)诱导的c-有丝分裂是主要畸变。强调了遗传毒性筛选在环境监测中相对于一般毒性检测的优势。关键词:葱试验，污染，遗传毒性，染色体，废水。环境科学，Vol.3(1&2) 2004: 55-58

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Global Journal of Environmental Sciences

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