The Differences of Electrophoretic Profile and Snake Venom Phospholipase A2 (svPLA2) Activity from the Venom of Javan Spitting Cobra, Naja sputatrix, based on Body Scales Color and Storage Condition

Abstract

: Naja sputatrix (Javan spitting cobra) is one of medically important snake species in Indonesia which have various dorsal scales color. This research purposes to examine the differences of venom general profile and its phospholipase A 2 (svPLA 2 ) activity from some N. sputatrix with different dorsal scales colors, and to examine the activity of N. sputatrix svPLA 2 in different storage conditions. A total of 6 N. sputatrix from East Java with various dorsal scales color were milked. Venom storage was performed at -80, 4 and 37°C in a maximum period of 14 days. Venom profile and phospholipase A 2 activity were examined through 15% SDS-PAGE and acidimetric method using egg yolk substrate respectively. Statistical analyses were performed to evaluate svPLA 2 activity in every dorsal color and storage condition. Few protein bands range in 16 – 22 kDa are only found in the venom of the certain dorsal color snake. Protein bands at 37°C were found to have the lightest intensity among other groups. The svPLA 2 activity of brown dorsal N. sputatrix is found as the highest activity. An interaction between the storage temperature factor and period factor has effects on post-storage svPLA 2 activity. Storage in 37°C effects on svPLA 2 activity declining compared to the control group and other experimental groups.