[Effect of DNA topoisomerase I inhibitor on the transduction efficiency of an deno-associated virus vector in human airway epithelial cells].

S. Teramoto, T. Matsuse, E. Ohga, H. Katayama, Y. Fukuchi, Y. Ouchi
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引用次数: 0

Abstract

We tested the effects of a DNA topoisomerase inhibitor (camptothecin; CPT) on the transduction efficiency of AAV vectors in cultured human airway epithelial cells. The cells were treated with CPT for 24 hours, then exposed to AAV-CMV-LacZ for 1 hour at different multiplicities of infection (moi). Transduction efficiency of AAV vectors was assessed using X-gal staining as the percentage of LacZ-expressing cells. The transduction efficiency was approximately 1.5 to 10 fold increased by treatment with CPT prior to AAV vector exposure. However, treatment with CPT after AAV vector infection did not enhance the transduction efficiency of the vectors. These results suggest that pre-treatment with CPT increases the transduction efficiency of AAV vectors, probably by nodulating cellular function.
[DNA拓扑异构酶I抑制剂对人气道上皮细胞中脱氧相关病毒载体转导效率的影响]。
我们测试了DNA拓扑异构酶抑制剂(喜树碱;CPT)对AAV载体在培养的人气道上皮细胞转导效率的影响。细胞用CPT处理24小时,然后在不同感染倍数(moi)下暴露于AAV-CMV-LacZ 1小时。用X-gal染色法测定表达lacz的细胞百分比,评估AAV载体的转导效率。在接触AAV载体之前,用CPT治疗的转导效率提高了约1.5至10倍。然而,AAV载体感染后用CPT治疗并没有提高载体的转导效率。这些结果表明,CPT预处理可能通过调节细胞功能来提高AAV载体的转导效率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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