Irene Carlberg , Kristina Glimelius , Tage Eriksson
{"title":"Nuclear DNA-content during the initiation of callus formation from isolated protoplasts of Solanum tuberosum L","authors":"Irene Carlberg , Kristina Glimelius , Tage Eriksson","doi":"10.1016/0304-4211(84)90233-5","DOIUrl":null,"url":null,"abstract":"<div><p>Cytofluorimetric measurements of the DNA-content in the nuclei during the initiation of callus formation from isolated protoplasts of potato were performed using propidium iodide as fluorochrome. Reproducible measurements were obtained after digestion of the cell walls and after RNase treatment.</p><p>Nuclei with high DNA-content were found early in the culture and the frequency of these nuclei increased with time in culture. In addition to highly replicated nuclei, multinucleated cells were found, the frequency of which decreased during prolonged culturing.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90233-5","citationCount":"49","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Science Letters","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0304421184902335","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 49
Abstract
Cytofluorimetric measurements of the DNA-content in the nuclei during the initiation of callus formation from isolated protoplasts of potato were performed using propidium iodide as fluorochrome. Reproducible measurements were obtained after digestion of the cell walls and after RNase treatment.
Nuclei with high DNA-content were found early in the culture and the frequency of these nuclei increased with time in culture. In addition to highly replicated nuclei, multinucleated cells were found, the frequency of which decreased during prolonged culturing.
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