Antiparkinson's and free radical scavenging study of ethyl acetate fraction of ethanolic extract of Leucas lanata

Abstract

Objective

Oxidative stress is the major reason for most of the neurodegenerative disorders. Our study aimed to know the in vitro free radical scavenging ability and in vivo antiparkinson's activity of ethyl acetate fraction of ethanolic extract of Leucas lanata (LLEA).

Methods

LLEA was initially screened for in vitro free radical scavenging ability and then the effect of LLEA was studied in rotenone induced Parkinson's disorder in mice.

Results

The IC₅₀ values for superoxide anion, hydroxyl radical and nitric oxide scavenging activity were 64.053 ± 11.90 μg/ml, 289.486 ± 66.57 μg/ml and 402.909 ± 43.22 μg/ml respectively. In catalepsy activity, fall of time for rotenone was found to be 39.5 ± 1.996 sec and it was decreased when animals were treated with LLEA (11.17 ± 0.6 sec). In locomotor activity, there was an increase from 40 ± 1.26 counts/5 min (rotenone) to 222.7 ± 1.87 counts/5 min (LLEA alone) and to 148.8 ± 1.95 counts/5 min (LLEA along with rotenone). In muscle relaxant activity, the fall off time was found to be increased from 43.83 ± 1.6 sec to 211.8 ± 1.22 sec and 147.3 ± 2.39 sec with LLEA alone and LLEA in combination with rotenone respectively.

Conclusion

The oxidative damage induced by rotenone was reduced by the administration of LLEA in all the concentrations linearly. The protective effect of LLEA might be due to its free radical scavenging ability. For the oxidative stress induced neurodegenerative disorders, natural herbs with free radical scavenging potential are going to be the better option of treatment.