Effects of thyroid hormone on the expression of homeobox gene Nkx6.1 in the cerebrum tissue of offspring of hypothyroidism rat

Q3 Medicine

中华地方病学杂志 Pub Date : 2010-03-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2010.02.011

N. Ren, Z. Rui, Wang Bei-lei, Li Jing-hua, Li Yuan, Liang Dong-chun, Guo Gang

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Abstract

Objective To explore the effects of thyroid hormone on the expression of homeobox gene Nkx6.1 in offspring of hypothyroidism rats and the relationship between gene expression and hormone level by supplying their hypothyroidism pregnant mother with thyroid hormone. Method A total of 240 Wistar rats were half nude and half female. Female rats were randomly divided into eight groups: control, hypothyroidism group, hypothyroidism groups which were supplied with thyroid hormone in high, medium and low dosage in early stage(1- 17 d) and in late stage( 18 - 20 d). According to 100 grams of body weight, the concentration of thyroid hormone were 3.5,2.0,0.5 μg/d in high, medium and low dosage group. All the rats were fed with low-iodine food. The normal control group was given KIO_3 solution and the other groups were given deionized water. After three months female rats were mated with male rats. The content of Nkx6.1 mRNA in brain tissue of 17-day fetal rats, new-born and 20- day old offspring by real-time fluorescence quantitative PCR techniques. Results①A rat model of hypothyroidism was successfully established, there were statistical significance between 8 groups in TT_3,TT_4,FT_3,FT_4(F=4.08,31.99,5.79,26.34, all P 0.05), while between the other 5 groups which were supplied with thyroid hormone and normal control group had significant difference(all P < 0.01 ) in the above three time points.⑦Multiple comparison of early stage groups which were supplied with thyroid hormone showed that the expression of Nkx6.1 mRNA had significant difference(all P < 0.01) between high, low dosage groups and medium group in 17-day fetal rats, new-bern and 20-day offspring(all P< 0.01). ⑧Multiple comparison of late stage groups supplied with thyroid hormone showed that old offspring and between high dosage groups and low dosage groups in 17-day fetal rats and 20-day the expression of Nkx6.1 mRNA had significant difference(all P < 0.01 ) between three groups in new-bern and 20- day old offspring. Conclusion The expression of Nkx6.1 in rats offspring is highly related to the supply dosage and supply time of thyroid hormone in hypothyroidism pregnant rats. Key words: Hypothyroidism; Homeobex gene; Thyroxine; Real-time fluorescence quantitative PCR

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甲状腺激素对甲状腺功能减退大鼠后代脑组织同源框基因Nkx6.1表达的影响

目的探讨甲状腺激素对甲状腺功能减退大鼠子代同源框基因Nkx6.1表达的影响，并探讨其与激素水平的关系。方法取Wistar大鼠240只，雌雄各占一半。将雌性大鼠随机分为8组:对照组、甲状腺功能减退组和甲状腺功能减退组，分别在早期(1 ~ 17 d)和晚期(18 ~ 20 d)给予高、中、低剂量甲状腺激素，高、中、低剂量组按100 g体重分别给予3.5、2.0、0.5 μg/d甲状腺激素。所有的老鼠都喂食低碘食物。正常对照组给予KIO_3溶液，其余各组给予去离子水。三个月后，雌性老鼠与雄性老鼠交配。采用实时荧光定量PCR技术检测17日龄胎鼠、新生仔鼠和20日龄仔鼠脑组织中Nkx6.1 mRNA的含量。结果①成功建立大鼠甲状腺功能减退模型，TT_3、TT_4、FT_3、FT_4 8组间差异均有统计学意义(F=4.08、31.99、5.79、26.34,P均0.05)，其余5组给予甲状腺激素与正常对照组间上述3个时间点差异均有统计学意义(P均< 0.01)。⑦早期给予甲状腺激素组的多次比较显示，高、低剂量组和中剂量组在17日龄胎鼠、新生仔鼠和20日龄仔鼠中Nkx6.1 mRNA的表达差异极显著(均P< 0.01)。⑧甲状腺激素晚期给药组的多重比较显示，17日龄胎鼠和20日龄胎鼠中Nkx6.1 mRNA的表达量、高剂量组和低剂量组之间的差异均有统计学意义(P < 0.01)。结论Nkx6.1在甲减妊娠大鼠子代中的表达与甲状腺激素的供给剂量和供给时间密切相关。关键词:甲状腺功能减退;Homeobex基因;甲状腺素;实时荧光定量PCR

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.