Review on Methods and Applications of High-Performance Liquid Chromatography

Abstract

High-Performance Liquid Chromatography is a technique used to separate components from the mixture. The technology consists of two phases, the stationary phase and the mobile phase. Component separation is based on differences in partition coefficients in two phases. HPLC is a form of liquid chromatography used to separate compounds dissolved in solution and qualitative and quantitative techniques used to evaluate pharmaceutical and biological samples. HPLC is the most widely used analytical separation technique for at quality control of pharmaceuticals. This mini-reviews include many aspects of HPLC, including type, instruments, and applications.